Microbial Gene Essentiality: Protocols and Bioinformatics

Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.

• Bur
• Escherichia coli
• Genomic databases
• In-silico prediction
• Metabolic models
• Microarray
• Microbial genome-scale essentiality
• Mutant
• Mutation
• PCR
• bioinformatics
• chromosome
• genes
• hybridization
• molecular biology

• Burnham Institute for Medical Research, La Jolla

  Andrei L. Osterman

• Fellowship for Interpretation of Genomes (FIG), Burr Ridge

  Svetlana Y. Gerdes

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