Methods in Molecular Biology

Microbial Gene Essentiality: Protocols and Bioinformatics

Editors: Osterman, Andrei L., Gerdes, Svetlana Y. (Eds.)

  • Offers a comprehensive, up-to-date collection of all the main strategies of experimental genome-scale gene essentiality analysis
  • Contains a detailed description of the procedures as well as a discussion of potential difficulties and failures
  • Includes chapters on statistical analysis of the generated datasets and their integration into existing genomic databases
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-59745-321-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
  • ISBN 978-1-58829-378-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA
  • ISBN 978-1-61737-529-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.

Table of contents (17 chapters)

  • Overview of Whole-Genome Essentiality Analysis

    Shaw, Karen Joy

    Pages 3-8

  • Transposon-Based Strategies for the Identification of Essential Bacterial Genes

    William S. Reznikoff, Kelly M. Winterberg

    Pages 13-26

  • Identification and Analysis of Essential Genes in Haemophilus influenzae

    Sandy M.S. Wong, Brian J. Akerley

    Pages 27-44

  • Transposon Site Hybridization in Mycobacterium tuberculosis

    Jeffrey P. Murry, Christopher M. Sassetti, James M. Lane, et al.

    Pages 45-59

  • Generating a Collection of Insertion Mutations in the Staphylococcus aureus Genome Using bursa aurealis

    Taeok Bae, Elizabeth M. Glass, Olaf Schneewind, et al.

    Pages 103-116

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-59745-321-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
  • ISBN 978-1-58829-378-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA
  • ISBN 978-1-61737-529-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Microbial Gene Essentiality: Protocols and Bioinformatics
Editors
  • Andrei L. Osterman
  • Svetlana Y. Gerdes
Series Title
Methods in Molecular Biology
Series Volume
416
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-59745-321-9
DOI
10.1007/978-1-59745-321-9
Hardcover ISBN
978-1-58829-378-7
Softcover ISBN
978-1-61737-529-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 486
Topics