Methods in Molecular Biology

Confocal Microscopy

Methods and Protocols

Editors: Paddock, Stephen W. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-60761-847-8
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Hardcover $139.00
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  • ISBN 978-1-58829-351-0
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About this book

Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques. 

Table of contents (18 chapters)

  • Reflecting on Confocal Microscopy: A Personal Perspective

    White, John

    Pages 1-7

  • Laser Scanning Confocal Microscopy: History, Applications, and Related Optical Sectioning Techniques

    Paddock, Stephen W. (et al.)

    Pages 9-47

  • Confocal Microscopy on the Internet

    Hazelwood, Kristin L. (et al.)

    Pages 49-96

  • Using Photoshop with Images Created by a Confocal System

    Sedgewick, Jerry

    Pages 97-128

  • Clearing Up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy

    Zimmermann, Timo (et al.)

    Pages 129-148

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-60761-847-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-58829-351-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5067-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Confocal Microscopy
Book Subtitle
Methods and Protocols
Editors
  • Stephen W. Paddock
Series Title
Methods in Molecular Biology
Series Volume
1075
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-60761-847-8
DOI
10.1007/978-1-60761-847-8
Hardcover ISBN
978-1-58829-351-0
Softcover ISBN
978-1-4939-5067-6
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 381
Number of Illustrations and Tables
79 b/w illustrations, 51 illustrations in colour
Topics