Overview
- Editors:
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John M. Walker
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University of Hertfordshire, Hatfield, UK
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Table of contents (73 protocols)
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Front Matter
Pages i-xviii
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- Brian Berg Vandahl, Gunna Christiansen, Svend Birkelund
Pages 19-26
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- Melinda L. Ramsby, Gregory S. Makowski
Pages 37-48
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- Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari, Craig D. Shriver
Pages 49-54
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- Darrell L. Ellsworth, Stephen Russell, Brenda Deyarmin, Anthony G. Sullivan, Henry Brzeski, Richard I. Somiari et al.
Pages 59-66
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- William L. Godfrey, Colette J. Rudd, Sujata Iyer, Diether Recktenwald
Pages 67-78
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- Régis Dieckmann, Yohann Couté, Denis Hochstrasser, Jean-Jacques Diaz, Jean-Charles Sanchez
Pages 79-85
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- Volker Badock, Albrecht Otto
Pages 87-95
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- Xun Zuo, Ki-Boom Lee, David W. Speicher
Pages 97-117
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- Henry Brzeski, Stephen Russell, Anthony G. Sullivan, Richard I. Somiari, Craig D. Shriver
Pages 119-131
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- Masamichi Oh-Ishi, Tadakazu Maeda
Pages 145-149
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- Sjouke Hoving, Hans Voshol, Jan van Oostrum
Pages 151-165
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- Steven E. Pfeiffer, Yoshihide Yamaguchi, Cecilia B. Marta, Rashmi Bansal, Christopher M. Taylor
Pages 167-175
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- Julia Poland, Thierry Rabilloud, Pranav Sinha
Pages 177-184
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About this book
Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Editors and Affiliations
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University of Hertfordshire, Hatfield, UK
John M. Walker