The Proteomics Protocols Handbook

Editors: Walker, John M. (Ed.)

Buy this book

eBook $259.00
price for USA (gross)
valid through November 5, 2017
  • ISBN 978-1-59259-890-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $329.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-58829-343-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $259.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-58829-593-4
  • Free shipping for individuals worldwide
  • This title is currently reprinting. You can pre-order your copy now.
About this book

Proteomics methodology has revolutionized the way that proteins are studied and opened new channels to understanding both cell functions and the cellular changes involved in disease states. In The Proteomics Protocols Handbook, hands-on researchers describe in step-by-step detail a wide range of proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques (73 in all) address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and posttranslational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein, including novel mass spectrometry and LC-MS techniques, protein array technology, and a variety of structural and functional proteomics techniques needed to determine of the function of newly discovered protein sequences. The use of X-ray crystallography and NMR to determine protein structure is also described. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and highly practical, The Proteomics Protocols Handbook offers molecular biologists, protein chemists, clinical and medicinal chemists, biochemists, and microbiologists a comprehensive collection of today's best tools for understanding not only the function of individual proteins in the cell, but also for identifying new therapeutic targets.

Table of contents (73 chapters)

  • Extraction and Solubilization of Proteins for Proteomic Studies

    Leimgruber, Richard M.

    Pages 1-18

  • Preparation of Bacterial Samples for 2-D PAGE

    Vandahl, Brian Berg (et al.)

    Pages 19-26

  • Preparation of Yeast Samples for 2-D PAGE

    Norbeck, Joakim

    Pages 27-30

  • Preparation of Mammalian Tissue Samples for Two-Dimensional Electrophoresis

    Witzmann, Frank A.

    Pages 31-35

  • Differential Detergent Fractionation of Eukaryotic Cells

    Ramsby, Melinda L. (et al.)

    Pages 37-48

Buy this book

eBook $259.00
price for USA (gross)
valid through November 5, 2017
  • ISBN 978-1-59259-890-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $329.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-58829-343-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $259.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-58829-593-4
  • Free shipping for individuals worldwide
  • This title is currently reprinting. You can pre-order your copy now.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
The Proteomics Protocols Handbook
Editors
  • John M. Walker
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-890-8
DOI
10.1385/1592598900
Hardcover ISBN
978-1-58829-343-5
Softcover ISBN
978-1-58829-593-4
Edition Number
1
Number of Pages
XVIII, 988
Topics