Methods in Molecular Biology

Epigenetics Protocols

Editors: Tollefsbol, Trygve (Ed.)

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About this book

Recent advances in epigenetic research as well as the development of exciting new technologies have helped greatly in unraveling the many mysteries of nontraditional genetic processes. In Epigenetics Protocols, hands-on researchers describe state-of-the-art methods for epigenetic analysis, including recent breakthrough techniques that have great potential in the rapidly expanding field of non-Mendelian genetics. The authors provide techniques for the analysis of chromatin remodeling, such as histone acetylation and methylation. In addition, methods in newly developed and especially promising areas of epigenetics, such as telomere position effects, quantitative epigenetics, and ADP ribosylation are covered. There is also an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure. Of special interest are potentially revolutionary techniques. These include methods for determining changes in native chromatin, methods of microarray analysis applied to epigenetics, and methylation-sensitive single-strand conformation techniques. The methods are suitable not only for studying fundamental biological processes, but also for investigating possible therapeutic interventions and such diseases as cancer. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easy to use, Epigenetics Protocols offers investigators readily reproducible techniques that will further promote progress in this critically important field.

Reviews

"Comprehensive and easy to use...offers investigators readily reproducible techniques that will further promote progress in this critically important field." - Tumori

Table of contents (22 chapters)

  • Methods of Epigenetic Analysis

    Tollefsbol, Trygve O.

    Pages 1-8

  • Chromatin Immunoprecipitation Assays

    Yan, Yan (et al.)

    Pages 9-19

  • Native Chromatin Immunoprecipitation

    Thorne, Alan W. (et al.)

    Pages 21-44

  • Q-PCR in Combination With ChIP Assays to Detect Changes in Chromatin Acetylation

    Irvine, Ryan A. (et al.)

    Pages 45-52

  • Restriction Endonuclease Accessibility as a Determinant of Altered Chromatin Structure

    Hempel, William M. (et al.)

    Pages 53-63

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-59259-828-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-58829-336-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA
  • ISBN 978-1-61737-501-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Epigenetics Protocols
Editors
  • Trygve Tollefsbol
Series Title
Methods in Molecular Biology
Series Volume
287
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-828-1
DOI
10.1385/1592598285
Hardcover ISBN
978-1-58829-336-7
Softcover ISBN
978-1-61737-501-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 304
Topics