Methods in Molecular Biology

Protein Arrays

Methods and Protocols

Editors: Fung, Eric (Ed.)

Buy this book

eBook n/a
  • ISBN 978-1-59259-759-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-58829-255-1
  • Free shipping for individuals worldwide
Softcover n/a
  • ISBN 978-1-61737-456-2
  • Free shipping for individuals worldwide
About this book

Protein arrays make possible the detection and quantitation of many proteins simultaneously, thus enabling researchers to ask fundamental questions about biological processes and to discover biomarkers that can be used diagnostically. In Protein Arrays: Methods and Protocols, innovative experimentalists describe in detail the methods they have developed to synthesize and construct protein arrays for basic and clinical research. The authors present protocols to create and immobilize the capture substrate-the first task in designing a protein array-using a variety of affinity capture reagents, including antibodies, peptides, aptamers, biotin, chemical reagents, and chromatographic substrates. Once synthesized, these arrays can be used to analyze protein-protein interactions and posttranslational modifications, such as phosphorylation, as well as to discover and characterize potential diagnostic markers. Protocols to accomplish these tasks are also presented. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Diverse and highly practical, Protein Arrays: Methods and Protocols offers basic and clinical investigators a broad spectrum of approaches to the generation of protein arrays, as well as their uses in biomarkers discovery, in assay development, in clinical sample testing, in signal transduction analysis and characterization, and for creating the next generation of molecular tools.

Reviews

"Diverse and highly practical...offers basic and clinical investigators a broad spectrum of approaches to the generation of protein arrays..." - Clinical Laboratory International

"...provides a broad spectrum of approaches for the generation, production and application of protein arrays." - BTi

Table of contents (23 chapters)

  • Protein Arrays From cDNA Expression Libraries

    Weiner, Hendrik (et al.)

    Pages 1-13

  • Protein Expression Arrays for Proteomics

    Gilbert, Michele (et al.)

    Pages 15-23

  • Generation of Protein In Situ Arrays by DiscernArray™ Technology

    He, Mingyue

    Pages 25-31

  • Multiplexed Protein Analysis Using Spotted Antibody Microarrays

    Haab, Brian B. (et al.)

    Pages 33-45

  • Target-Assisted Iterative Screening of Phage Surface Display cDNA Libraries

    Kurakin, Alexei (et al.)

    Pages 47-60

Buy this book

eBook n/a
  • ISBN 978-1-59259-759-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-58829-255-1
  • Free shipping for individuals worldwide
Softcover n/a
  • ISBN 978-1-61737-456-2
  • Free shipping for individuals worldwide
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Protein Arrays
Book Subtitle
Methods and Protocols
Editors
  • Eric Fung
Series Title
Methods in Molecular Biology
Series Volume
264
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-759-8
DOI
10.1385/1592597599
Hardcover ISBN
978-1-58829-255-1
Softcover ISBN
978-1-61737-456-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 287
Topics