Methods in Molecular Biology

Genetic Recombination

Reviews and Protocols

Editors: Waldman, Alan S. (Ed.)

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About this book

Genetic recombination plays an essential role in such vitally important biological processes as DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. In Genetic Recombination: Reviews and Protocols, prominent international investigators actively engaged in recombination research describe in great detail their best techniques for studying recombination. The methods range from approaches and model systems to be used in a variety of eukaryotic organisms and in a mammalian parasite, to biochemical analyses useful for furthering the understanding of recombination mechanisms. Additional techniques use recombination as a reporter of genomic instability in lower and higher eukaryotes and as a tool for producing targeted genetic modification. Each readily reproducible method includes step-by-step instructions, a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Genetic Recombination: Reviews and Protocols offers both novice and experienced investigators an illuminating synthesis of what we currently know about recombination, as well as a wide-ranging collection of powerful tools for elucidating its mechanisms and regulation throughout biology today.

Table of contents (15 chapters)

  • Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae

    Spell, Rachelle Miller (et al.)

    Pages 3-12

  • Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells

    Smith, Jason A. (et al.)

    Pages 13-23

  • Intrachromosomal Homologous Recombination in Arabidopsis thaliana

    Schmidt-Puchta, Waltraud (et al.)

    Pages 25-34

  • Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-SceI Nuclease

    Nickoloff, Jac A. (et al.)

    Pages 35-52

  • Transformation of Monomorphic and Pleomorphic Trypanosoma brucei

    McCulloch, Richard (et al.)

    Pages 53-86

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-59259-761-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-58829-236-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-1-61737-442-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Genetic Recombination
Book Subtitle
Reviews and Protocols
Editors
  • Alan S. Waldman
Series Title
Methods in Molecular Biology
Series Volume
262
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-59259-761-1
DOI
10.1385/1592597610
Hardcover ISBN
978-1-58829-236-0
Softcover ISBN
978-1-61737-442-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 260
Topics