Methods in Molecular Biology

Oligonucleotide Synthesis

Methods and Applications

Editors: Herdewijn, Piet (Ed.)

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About this book

Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today.

Table of contents (25 chapters)

  • Synthesis of DNA Using a New Two-Step Cycle

    Dellinger, Douglas J. (et al.)

    Pages 1-16

  • RNA Synthesis Using 2′-O-(Tert-Butyldimethylsilyl) Protection

    Sproat, Brian S.

    Pages 17-31

  • RNA Oligonucleotide Synthesis Via 5′-Silyl-2′-Orthoester Chemistry

    Hartsel, Stephanie A. (et al.)

    Pages 33-49

  • Dimethylthiarum Disulfide

    Wang, Zhiwei (et al.)

    Pages 51-63

  • Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes

    Galarneau, Annie (et al.)

    Pages 65-80

Buy this book

eBook $159.00
price for USA (gross)
  • ISBN 978-1-59259-823-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
  • ISBN 978-1-58829-233-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $209.00
price for USA
  • ISBN 978-1-61737-441-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Oligonucleotide Synthesis
Book Subtitle
Methods and Applications
Editors
  • Piet Herdewijn
Series Title
Methods in Molecular Biology
Series Volume
288
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-823-6
DOI
10.1385/1592598234
Hardcover ISBN
978-1-58829-233-9
Softcover ISBN
978-1-61737-441-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 436
Number of Illustrations and Tables
142 b/w illustrations
Topics