Methods in Molecular Biology

Antibody Engineering

Methods and Protocols

Editors: Lo, Benny K. C. (Ed.)

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About this book

With 10 recent FDA approvals of therapeutic antibody drugs and hundreds more in the biotechnology pipeline, it is clear that engineered antibodies will play a major role in treating human diseases in the 21st century. In Antibody Engineering: Methods and Protocols, leading researchers and clinicians present a core collection of cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the generation of human recombinant antibodies by humanization approaches, molecular display technologies, and transgenic animals. The techniques for antibody expression and optimization utilize not only bacterial and mammalian cell cultures, but also insect cell cultures and transgenic plants. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics. Each fully tested protocol is described in step-by-step detail and includes background material, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls.
Comprehensive and up-to-date, Antibody Engineering: Methods and Protocols provides both laboratory and clinical investigators with a diverse array of the fundamental techniques upon which new and imaginative drug technologies can be successfully developed.

Reviews

"...as the book provides as comprehensive collection of clear, detailed methods it is very likely to prove its worth as a laboratory reference." - Immunology News

"...a valuable addition to in-lab books" - Microbiology Today

Table of contents (32 chapters)

  • Internet Resources for the Antibody Engineer

    Lo, Benny K. C. (et al.)

    Pages 3-9

  • The Kabat Database and a Bioinformatics Example

    Johnson, George (et al.)

    Pages 11-25

  • IMGT, The International ImMunoGeneTics Information System®, http://imgt.cines.fr

    Lefranc, Marie-Paule

    Pages 27-49

  • Antibody Variable Regions

    Whitelegg, Nicholas (et al.)

    Pages 51-91

  • Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling

    Harding, Stephen E. (et al.)

    Pages 93-113

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-59259-666-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
  • ISBN 978-1-58829-092-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA
  • ISBN 978-1-61737-352-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Antibody Engineering
Book Subtitle
Methods and Protocols
Editors
  • Benny K. C. Lo
Series Title
Methods in Molecular Biology
Series Volume
248
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-666-9
DOI
10.1385/1592596665
Hardcover ISBN
978-1-58829-092-2
Softcover ISBN
978-1-61737-352-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 562
Topics