Methods in Molecular Biology

Protein Kinase C Protocols

Editors: Newton, Alexandra C. (Ed.)

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About this book

Protein kinase C impacts on nearly every aspect of signal transduction, placing it at the forefront of research in how cells transduce information. In Protein Kinase C Protocols, leading investigators from academia and industry present a comprehensive collection of classical and emerging laboratory techniques for the study of this influential protein, all optimized for successful and reproducible results. Described in step-by-step detail, these methods can be easily used to explore the structure, function, regulation, subcellular localization, and macromolecular interactions of protein kinase C. Included in this collection are easy-to-follow techniques to purify protein kinase C, to measure its activity and its membrane interaction in vivo and in vitro, and to identify substrates. There are also methodologies and techniques for measuring the phosphorylation state of protein kinase C, for expressing and analyzing the membrane-targeting domains of protein kinase C, and for detecting binding proteins. Additional chapters address pharmacological probes, genetic approaches, and protein kinase C in disease. Each protocol is introduced in the context of protein kinase C function and regulation and contains many notes on how best to deal with the problems that may occur.
Comprehensive and authoritative, Protein Kinase C Protocols offers a timely compilation of biophysical, biochemical, cell biological, and molecular biological approaches that will ensure successful results in every laboratory working in this rapidly advancing field.

Reviews

"...a comprehensive collection of established and emerging techniques used for the study of protein kinase C, and is quite simply an essential guide for any lab interested in cell signallin...an impressive collection of expert contributors...this is an excellent lab manual which provides very detailed and easy to follow methods and instructions that would be useful for any lab that is interested in any form of cell signalling..." - Proteomics

"...a valuable book for everybody interested in PKC, and it is a must for everybody doing research with it." - ChemBioChem

Table of contents (42 chapters)

  • The Ins and Outs of Protein Kinase C

    Newton, Alexandra C.

    Pages 3-7

  • Early Studies of Protein Kinase C

    Nishizuka, Yasutomi (et al.)

    Pages 9-18

  • Expression and Purification of Protein Kinase C from Insect Cells

    Mukai, Hideyuki (et al.)

    Pages 21-34

  • Expression and Purification of Protein Kinase Cδ from Bacteria

    Gschwendt, Michael (et al.)

    Pages 35-42

  • Complexities in Protein Kinase C Activity Assays

    Sando, Julianne J.

    Pages 45-61

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-59259-397-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-58829-068-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-61737-335-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Kinase C Protocols
Editors
  • Alexandra C. Newton
Series Title
Methods in Molecular Biology
Series Volume
233
Copyright
2003
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-397-2
DOI
10.1385/1592593976
Hardcover ISBN
978-1-58829-068-7
Softcover ISBN
978-1-61737-335-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 584
Topics