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Transgenic Cotton

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Baohong Zhang

    1. Department of Biology, East Carolina University, Greenville, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1902)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
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  4. Genome Editing and Vigs

    1. Front Matter

      Pages 93-93
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    2. Genome Editing in Cotton Using CRISPR/Cas9 System

      • Chao Li, Baohong Zhang
      Pages 95-104

    3. Tobacco Rattle Virus-Induced Gene Silencing in Cotton

      • Caiping Cai, Xinyu Wang, Baohong Zhang, Wangzhen Guo
      Pages 105-119

  5. Detection

    1. Front Matter

      Pages 121-121
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    2. Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome

      • Jun Zhang, Yan Hong
      Pages 123-136

    3. Estimating the Copy Number of Transgenes in Transformed Cotton by Real-Time Quantitative PCR

      • Chengxin Yi, Yan Hong
      Pages 137-157

    4. Development of an Enzyme-Linked Immunosorbent Assay for the Detection of GM Proteins in Transgenic Crops/Produce

      • Suchitra Kamle, Dawei Li, Abhishek Ojha, Arvind Kumar
      Pages 159-166

    5. Screening of Transgenic Cotton Based on a Porous Silicon Biosensor

      • Liangliang Chen, Peng Li, Xiaoyi Lv, Ji Ma
      Pages 167-176

    6. YC3.60-Based Imaging Analysis on Calcium Level in Cotton Cells

      • Mi Zhang, Jianyan Zeng, Huizhen Cao, Yan Pei
      Pages 177-185

    7. A Simple and Rapid Method for Determining Transgenic Cotton Plants Using a Marker Gene

      • Baohong Zhang, Hongmei Wang, Fang Liu, Qinglian Wang
      Pages 187-194
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Keywords

About this book

This second edition provides a comprehensive collection of the cutting-edge methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Chapters detail current status and perspectives of transgenic cotton, principle and methods for making transgenic cotton, creating gene knockout lines, foreign gene copy and expression in transgenic plants, improvements to cotton using transgenic technology, and monitoring the potential impact of transgenic cotton on environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and cutting-edge, Transgenic Cotton: Methods and Protocols 2nd aims to be a resource for scientists as well as graduate students who work on transgenic plants, plant genetics, molecular biology, and agricultural sciences.

Editors and Affiliations

  • Department of Biology, East Carolina University, Greenville, USA

    Baohong Zhang

Bibliographic Information

  • Book Title: Transgenic Cotton

  • Book Subtitle: Methods and Protocols

  • Editors: Baohong Zhang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8952-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8951-5Published: 13 December 2018

  • eBook ISBN: 978-1-4939-8952-2Published: 12 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 327

  • Number of Illustrations: 29 b/w illustrations, 34 illustrations in colour

  • Topics: Plant Sciences, Transgenics

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