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Chronic Lymphocytic Leukemia

Methods and Protocols

Editors:

  • Include cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implemenation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1881)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
  2. Ex-Vivo Signal Transduction Studies in Chronic Lymphocytic Leukemia

    • Karly-Rai Rogers-Broadway, Laura I. Karydis, Rachel C. Dobson, Andrew J. Steele
    Pages 1-17
  3. Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells

    • Burcu Aslan, Mary L. Ayres, Varsha Gandhi
    Pages 19-25
  4. Immunoglobulin Gene Analysis in Chronic Lymphocytic Leukemia

    • Andreas Agathangelidis, Richard Rosenquist, Frederic Davi, Paolo Ghia, Chrysoula Belessi, Anastasia Hadzidimitriou et al.
    Pages 51-62
  5. Detection and Functional Analysis of TP53 Mutations in CLL

    • Sarka Pavlova, Jana Smardova, Nikola Tom, Martin Trbusek
    Pages 63-81
  6. The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-Mutant CLL

    • Tushar Murthy, Kiran V. Paul, Alexander C. Minella, Manoj M. Pillai
    Pages 83-99
  7. Methods for Investigating VLA-4 (CD49d/CD29) Expression and Activation in Chronic Lymphocytic Leukemia and Its Clinical Applications

    • Antonella Zucchetto, Erika Tissino, Alexander Chigaev, Tanja Nicole Hartmann, Valter Gattei
    Pages 101-112
  8. In Vitro Assay to Study CLL and Monocyte Interactions

    • Maria Teresa Sabrina Bertilaccio, Ronghua Zhang, Priyanka Banerjee, Varsha Gandhi
    Pages 113-119
  9. Measurement of Leukemic B-Cell Growth Kinetics in Patients with Chronic Lymphocytic Leukemia

    • Andrea N. Mazzarello, Mark Fitch, Marc K. Hellerstein, Nicholas Chiorazzi
    Pages 129-151
  10. Assays on DNA Damage and Repair in CLL

    • Tzung-Huei Lai, Deepa Sampath
    Pages 153-163
  11. Immunological Methods to Study Monoclonal Antibody Activity in Chronic Lymphocytic Leukaemia

    • M. J. Carter, L. N. Dahal, K. L. S. Cleary, M. J. E. Marshall, R. R. French, S. A. Beers et al.
    Pages 173-184
  12. CRISPR/Cas9-Based Gene Dropout Screens

    • Kai Wu, Sami N. Malek
    Pages 185-200
  13. Gene Disruption Using CRISPR-Cas9 Technology

    • Nan Hu, Sami N. Malek
    Pages 201-209
  14. Methods for Measuring ctDNA in Lymphomas

    • Davide Rossi, Adalgisa Condoluci, Valeria Spina, Gianluca Gaidano
    Pages 253-265

About this book

This book aims to provide scientists with tools and well-researched protocols to enable their research and to facilitate further progress in this common leukemia. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Chronic Lymphocytic Leukemia: Methods and Protocols aims to accelerate research on chronic lymphocytic leukemia and further improvements in patient outcomes. 

Editors and Affiliations

  • Department of Internal Medicine, University of Michigan, Ann Arbor, USA

    Sami N. Malek

Bibliographic Information

  • Book Title: Chronic Lymphocytic Leukemia

  • Book Subtitle: Methods and Protocols

  • Editors: Sami N. Malek

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8876-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8875-4Published: 22 October 2018

  • Softcover ISBN: 978-1-4939-9405-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8876-1Published: 22 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 373

  • Number of Illustrations: 7 b/w illustrations, 37 illustrations in colour

  • Topics: Cancer Research

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access