Skip to main content
SpringerLink
Log in

Influenza Virus

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Yohei Yamauchi

    1. School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of the influenza virus
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1836)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Understanding Influenza

    • Edward C. Hutchinson, Yohei Yamauchi
    Pages 1-21

  3. Clinical Diagnosis of Influenza

    • Yoshinori Ito
    Pages 23-31

  4. Influenza A Virus Genetic Tools: From Clinical Sample to Molecular Clone

    • Stéphanie Anchisi, Ana Rita Gonçalves, Béryl Mazel-Sanchez, Samuel Cordey, Mirco Schmolke
    Pages 33-58

  5. Propagation and Titration of Influenza Viruses

    • Umut Karakus, Michel Crameri, Caroline Lanz, Emilio Yángüez
    Pages 59-88

  6. Purification and Proteomics of Influenza Virions

    • Edward C. Hutchinson, Monika Stegmann
    Pages 89-120

  7. Haploid Screening for the Identification of Host Factors in Virus Infection

    • Evelyn Fessler, Lucas T. Jae
    Pages 121-137

  8. Phenotypic Lentivirus Screens to Identify Antiviral Single Domain Antibodies

    • Florian Ingo Schmidt
    Pages 139-158

  9. Deciphering Virus Entry with Fluorescently Labeled Viral Particles

    • Anja B. Hoffmann, Magalie Mazelier, Psylvia Léger, Pierre-Yves Lozach
    Pages 159-183

  10. Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape

    • Wen-Chi Su, Michael M. C. Lai
    Pages 185-194

  11. Single-Molecule Sensitivity RNA FISH Analysis of Influenza Virus Genome Trafficking

    • Yi-ying Chou, Timothée Lionnet
    Pages 195-211

  12. 3D Electron Microscopy (EM) and Correlative Light Electron Microscopy (CLEM) Methods to Study Virus-Host Interactions

    • Inés Romero-Brey
    Pages 213-236

  13. Correlative Light and Electron Microscopy of Influenza Virus Entry and Budding

    • Lorna Hodgson, Paul Verkade, Yohei Yamauchi
    Pages 237-260

  14. Influenza Virus-Liposome Fusion Studies Using Fluorescence Dequenching and Cryo-electron Tomography

    • Long Gui, Kelly K. Lee
    Pages 261-279

  15. Metal-Tagging Transmission Electron Microscopy and Immunogold Labeling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging

    • Martin Sachse, Isabel Fernández de Castro, Guillaume Fournier, Nadia Naffakh, Cristina Risco
    Pages 281-301

  16. Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy

    • Amar R. Bhagwat, Valerie Le Sage, Seema S. Lakdawala
    Pages 303-327

  17. Purification of Unanchored Polyubiquitin Chains from Influenza Virions

    • Yasuyuki Miyake, Patrick Matthias, Yohei Yamauchi
    Pages 329-342

  18. Assays to Measure the Activity of Influenza Virus Polymerase

    • Aartjan J. W. te Velthuis, Jason S. Long, Wendy S. Barclay
    Pages 343-374

  19. In Vitro Models to Study Influenza Virus and Staphylococcus aureus Super-Infection on a Molecular Level

    • Christin Bruchhagen, Andre van Krüchten, Carolin Klemm, Stephan Ludwig, Christina Ehrhardt
    Pages 375-386

  20. Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus

    • Hannah M. Creager, Terrence M. Tumpey, Taronna R. Maines, Jessica A. Belser
    Pages 387-399
Back to top

Keywords

About this book

This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, biochemistry, viral transcription, physiological infection models, animal models, in vivo imaging, antigenic evolution, immunology to mathematical modelling. Reviews cover general influenza, clinical trials, both sides of the gain-of-function debate, and computational modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Influenza Virus: Methods and Protocols aims to motivate experienced researchers and newcomers in the field and improve our overall understanding of influenza.

Editors and Affiliations

  • School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

    Yohei Yamauchi

Bibliographic Information

  • Book Title: Influenza Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Yohei Yamauchi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8678-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8677-4Published: 28 August 2018

  • Softcover ISBN: 978-1-4939-9363-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8678-1Published: 27 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 663

  • Number of Illustrations: 23 b/w illustrations, 160 illustrations in colour

  • Topics: Infectious Diseases

Publish with us

Policies and ethics

Back to top

Search

Navigation