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Lung Innate Immunity and Inflammation

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Scott Alper

    1. Department of Immunology and Microbiology University of Colorado School of Medicine, Department of Biomedical Research and Center for Genes Environment and Health, National Jewish Health Denver, CO, USA, Aurora,, USA

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  2. William J. Janssen

    1. Division of Pulmonary Sciences and Critical Care Medicine Department of Medicine University of Colorado Denver, Division of Pulmonary, Critical Care and Sleep Medicine Department of Medicine National Jewish Health Denver, CO, USA, Aurora, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1809)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Lung Innate, Immunity, and Inflammation

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. 500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy

      • William McKleroy, Kenneth Lyn-Kew
      Pages 3-15

    3. Overview of Innate Lung Immunity and Inflammation

      • David W. H. Riches, Thomas R. Martin
      Pages 17-30

  3. Isolation and Characterization of Lung Innate Immune Cells

    1. Front Matter

      Pages 31-31
      Download chapter PDF

    2. Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes

      • Sophie L. Gibbings, Claudia V. Jakubzick
      Pages 33-44

    3. Isolation and Characterization of Mouse Neutrophils

      • Niki D. J. Ubags, Benjamin T. Suratt
      Pages 45-57

    4. Isolation and Characterization of Mouse Fibroblasts

      • Benjamin L. Edelman, Elizabeth F. Redente
      Pages 59-67

    5. Isolation of Rat and Mouse Alveolar Type II Epithelial Cells

      • Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok et al.
      Pages 69-82

    6. Isolation and Characterization of Human Alveolar Type II Cells

      • Beata Kosmider, Robert J. Mason, Karim Bahmed
      Pages 83-90

    7. Air–Liquid Interface Culture of Human and Mouse Airway Epithelial Cells

      • Di Jiang, Niccolette Schaefer, Hong Wei Chu
      Pages 91-109

    8. Isolation and Characterization of Human Lung Myeloid Cells

      • Yen-Rei A. Yu, Robert M. Tighe
      Pages 111-119

    9. Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells Under Pathological Conditions

      • Xiaohui Fang, Michael A. Matthay
      Pages 121-128

    10. Measuring Innate Immune Function in Mouse Mononuclear Phagocytes

      • John Matthew Craig, Neil Raj Aggarwal
      Pages 129-137

    11. Measuring Neutrophil Bactericidal Activity

      • Kenneth C. Malcolm
      Pages 139-144

    12. Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches

      • Alexandra L. McCubbrey, William J. Janssen
      Pages 145-168

    13. Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches

      • Adrianne L. Stefanski, Dorota S. Raclawska, Christopher M. Evans
      Pages 169-201

    14. Computational Analysis of RNA-Seq Data from Airway Epithelial Cells for Studying Lung Disease

      • Nathan D. Jackson, Lando Ringel, Max A. Seibold
      Pages 203-235

    15. Application of Proteomics in Lung Research

      • Nichole A. Reisdorph, Cole Michel, Kristofer Fritz, Richard Reisdorph
      Pages 237-262

    16. Application of Metabolomics in Lung Research

      • Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, Richard Reisdorph
      Pages 263-288
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Keywords

About this book

This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.


Editors and Affiliations

  • Department of Immunology and Microbiology University of Colorado School of Medicine, Department of Biomedical Research and Center for Genes Environment and Health, National Jewish Health Denver, CO, USA, Aurora,, USA

    Scott Alper

  • Division of Pulmonary Sciences and Critical Care Medicine Department of Medicine University of Colorado Denver, Division of Pulmonary, Critical Care and Sleep Medicine Department of Medicine National Jewish Health Denver, CO, USA, Aurora, USA

    William J. Janssen

Bibliographic Information

  • Book Title: Lung Innate Immunity and Inflammation

  • Book Subtitle: Methods and Protocols

  • Editors: Scott Alper, William J. Janssen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8570-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8569-2Published: 09 July 2018

  • Softcover ISBN: 978-1-4939-9329-1Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8570-8Published: 09 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 433

  • Number of Illustrations: 21 b/w illustrations, 70 illustrations in colour

  • Topics: Molecular Medicine

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