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Cellulases

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Mette Lübeck

    1. Section of Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University, Copenhagen, Denmark

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1796)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Introduction Chapter and a Case Example

    1. Front Matter

      Pages 1-1
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    2. Cellulases: Role in Lignocellulosic Biomass Utilization

      • Sanjeev Kumar Soni, Amita Sharma, Raman Soni
      Pages 3-23

    3. Discovery of a Novel Fungus with an Extraordinary β-Glucosidase and Potential for On-Site Production of High Value Products

      • Peter Stephensen Lübeck, Mette Lübeck
      Pages 25-33

  3. Protocols

    1. Front Matter

      Pages 35-35
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    2. Isolation and Screening of Cellulolytic Filamentous Fungi

      • Mette Lübeck, Peter Stephensen Lübeck
      Pages 37-45

    3. Isolation and Screening of Cellulose-Degrading Microorganisms from Different Ecological Niches

      • Ayyappa Kumar Sista Kameshwar, Wensheng Qin
      Pages 47-56

    4. Isolation of Cellulolytic Bacteria from the Rumen

      • Makoto Mitsumori
      Pages 57-65

    5. Methods for Discovery of Novel Cellulosomal Cellulases Using Genomics and Biochemical Tools

      • Yonit Ben-David, Bareket Dassa, Lizi Bensoussan, Edward A. Bayer, Sarah Moraïs
      Pages 67-84

    6. Metatranscriptomic Techniques for Identifying Cellulases in Termites and their Symbionts

      • Brittany F. Peterson, Michael E. Scharf
      Pages 85-101

    7. Discovery of Novel Cellulases Using Proteomic Strategies

      • Marta Zoglowek, Heather Brewer, Angela Norbeck
      Pages 103-113

    8. Identification of Key Components for the Optimization of Cellulase Mixtures Using a Proteomic Strategy

      • Jingyao Qu, Jing Zhu, Guodong Liu, Yinbo Qu
      Pages 115-122

    9. Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger

      • Morgann C. Reilly, Saori Amaike Campen, Blake A. Simmons, Scott E. Baker, John M. Gladden, Jon K. Magnuson
      Pages 123-133

    10. Advanced Cloning Tools for Construction of Designer Cellulosomes

      • Amaranta Kahn, Edward A. Bayer, Sarah Moraïs
      Pages 135-151

    11. Evaluation of Thermal Stability of Cellulosomal Hydrolases and Their Complex Formation

      • Amaranta Kahn, Anastasia P. Galanopoulou, Dimitris G. Hatzinikolaou, Sarah Moraïs, Edward A. Bayer
      Pages 153-166

  4. Assays for Activity Testing

    1. Front Matter

      Pages 167-167
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    2. Endoglucanase (EG) Activity Assays

      • M. Shafiqur Rahman, Sheran Fernando, Brian Ross, Jiangning Wu, Wensheng Qin
      Pages 169-183

    3. Cellobiohydrolase (CBH) Activity Assays

      • Hem Kanta Sharma, Wensheng Qin, Chunbao (Charles) Xu
      Pages 185-199

    4. Two-Dimensional High-Throughput Endo-Enzyme Screening Assays Based on Chromogenic Polysaccharide Hydrogel and Complex Biomass Substrates

      • Julia Schückel, Stjepan Krešimir Kračun
      Pages 201-217

    5. Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs)

      • Bjørge Westereng, Jennifer S. M. Loose, Gustav Vaaje-Kolstad, Finn L. Aachmann, Morten Sørlie, Vincent G. H. Eijsink
      Pages 219-246
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Keywords

About this book

This volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for novel fungal b-glucosidases in a variety of fungal strains; discovery of novel enzymes in bacteria or fungi; protocols for isolating cellulolytic rumen bacteria; analysis of enzyme mixtures produced by ligocellulolytic fungi; and homology modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cellulases: Methods and Protocols is a valuable resource for biochemists, molecular biologists, chemical engineers, and industrial researchers interested in this field.



Editors and Affiliations

  • Section of Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University, Copenhagen, Denmark

    Mette Lübeck

Bibliographic Information

  • Book Title: Cellulases

  • Book Subtitle: Methods and Protocols

  • Editors: Mette Lübeck

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7877-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7876-2Published: 01 June 2018

  • Softcover ISBN: 978-1-4939-9312-3Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7877-9Published: 31 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 324

  • Number of Illustrations: 17 b/w illustrations, 41 illustrations in colour

  • Topics: Biochemistry, general

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