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Myelin

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1791)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
  2. Imaging Myelin and Biochemical Analysis

    1. Front Matter

      Pages 1-1
    2. Electron Microscopy for the Analysis of Peripheral Nerve Myelin

      • Cinzia Ferri, Angelo Quattrini, Maurizio D’Antonio
      Pages 3-13
    3. Immunohistochemical Analysis of Myelin Structures

      • M. Thetiot, S. A. Freeman, A. Desmazières
      Pages 15-23
    4. Lipidomics Profiling of Myelin

      • Chunyan Wang, Juan Pablo Palavicini, Xianlin Han
      Pages 37-50
    5. Mapping Lipid Distribution in Rat Sciatic Nerve Using Imaging Mass Spectrometry

      • Roberto Fernández, Jone Garate, Beatriz Abad, Begoña Ochoa, José A. Fernández
      Pages 51-65
    6. Proteomics Analysis of Myelin Composition

      • Akihiro Ishii, David Han, Rashmi Bansal
      Pages 67-77
  3. Cell Culture Systems

    1. Front Matter

      Pages 79-79
    2. Isolation and Purification of Primary Rodent Schwann Cells

      • Marta Palomo Irigoyen, Miguel Tamayo Caro, Encarnacion Pérez Andrés, Adrián Barreira Manrique, Marta Varela Rey, Ashwin Woodhoo
      Pages 81-93
    3. Isolation, Expansion, and Maturation of Oligodendrocyte Lineage Cells Obtained from Rat Neonatal Brain and Optic Nerve

      • Maria Victoria Sánchez-Gómez, Mari Paz Serrano, Elena Alberdi, Fernando Pérez-Cerdá, Carlos Matute
      Pages 95-113
    4. DRG Neuron/Schwann Cells Myelinating Cocultures

      • Carla Taveggia, Alessandra Bolino
      Pages 115-129
    5. Neuron/Oligodendrocyte Myelination Coculture

      • Yi Pang, Kimberly Simpson, José Javier Miguel-Hidalgo, Renate Savich
      Pages 131-144
    6. Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination

      • Amin Sherafat, Robert A. Hill, Akiko Nishiyama
      Pages 145-156
    7. Modeling PNS and CNS Myelination Using Microfluidic Chambers

      • Adrien Vaquié, Alizée Sauvain, Claire Jacob
      Pages 157-168
    8. Modeling CNS Myelination Using Micropillar Arrays

      • Nan-Xing Huang, Yun-An A. Shen, Feng Mei
      Pages 169-177
    9. A Central Nervous System Axonal Myelination Assay for High-Throughput Screening

      • Karen Lariosa-Willingham, Dmitri Leonoudakis
      Pages 179-192
    10. Analyzing Autophagic Flux in Nerve Cultures

      • Miguel Tamayo Caro, Marta Palomo Irigoyen, Encarnación Pérez Andrés, Adrián Barreira Manrique, Marta Varela Rey, Ashwin Woodhoo
      Pages 193-206
  4. Modeling Demyelinating Disorders and In Vivo Targeting Strategies

    1. Front Matter

      Pages 225-225

About this book

This volume collects research methodologies for both basic and translational studies of myelin authored by experts in the field. Chapters in this book are divided into three parts: Part One focuses on visualizing myelin and studying it on a biochemical level; Part Two looks at cell culture systems used to uncover key mechanisms that regulate formation and maintenance of myelin; and Part Three discusses modeling disorders of myelin in both CNS and PNS, and therapeutic strategies for targeting these disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Myelin: Methods and Protocols is a valuable resource for physiologists, molecular biologists, anatomists, cell and developmental biologists, and physician-scientists working in the field of myelin biology and the disorders associated with it.



Editors and Affiliations

  • Nerve Disorders Laboratory, CIC bioGUNE, Derio, Spain

    Ashwin Woodhoo

  • Basque Foundation for Science, IKERBASQUE, Bilbao, Spain

    Ashwin Woodhoo

Bibliographic Information

  • Book Title: Myelin

  • Book Subtitle: Methods and Protocols

  • Editors: Ashwin Woodhoo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7862-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7861-8Published: 14 July 2018

  • Softcover ISBN: 978-1-4939-9307-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7862-5Published: 13 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 294

  • Number of Illustrations: 26 b/w illustrations, 44 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access