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Epitope Mapping Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Johan Rockberg

    1. KTH School of Engineering Sciences in Chemistry, Biotechnology and Health, Protein Technology, Stockholm, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Johan Nilvebrant

    1. Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1785)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. An Introduction to Epitope Mapping

    • Johan Nilvebrant, Johan Rockberg
    Pages 1-10

  3. Whole Antigen Methods

    1. Front Matter

      Pages 11-11
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    2. Epitope Mapping of Antibody-Antigen Interactions with X-Ray Crystallography

      • Moeko Toride King, Cory L. Brooks
      Pages 13-27

    3. Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations

      • Luca Simonelli, Mattia Pedotti, Marco Bardelli, Simon Jurt, Oliver Zerbe, Luca Varani
      Pages 29-51

    4. Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors

      • Diluka Peiris, Teodor Aastrup, Samuel Altun, Camilla Käck, Maria Gianneli, Davide Proverbio et al.
      Pages 53-63

    5. Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically Encoded Photo-Activatable Cross-Linkers

      • Thomas Huber, Thomas P. Sakmar
      Pages 65-75

    6. Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing

      • Tariq Ahmad Najar, Shruti Khare, Raghavan Varadarajan
      Pages 77-88

    7. Epitope Mapping Using Yeast Display and Next Generation Sequencing

      • Thomas Van Blarcom, Andrea Rossi, Davide Foletti, Purnima Sundar, Steven Pitts, Zea Melton et al.
      Pages 89-118

  4. Protein Domain and Fragment-Based Methods

    1. Front Matter

      Pages 119-119
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    2. B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent Assay

      • Thiru Vanniasinkam, Mary D. Barton, Tongted Phumoonna Das, Michael W. Heuzenroeder
      Pages 121-128

    3. Array-In-Well Epitope Mapping of Phage-Displayed Antibodies

      • Urpo Lamminmäki, Gaurav Batra, Petri Saviranta
      Pages 129-140

    4. Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries

      • Anna-Luisa Volk, Francis Jingxin Hu, Johan Rockberg
      Pages 141-157

    5. Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains

      • Anna-Luisa Volk, Johan Rockberg
      Pages 159-183

  6. Antibody Specificity and T-Cell Prediction

    1. Front Matter

      Pages 221-221
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    2. Antibody Specificity Profiling Using Protein Microarrays

      • Pedro Ramos-López, José Irizarry, Ignacio Pino, Seth Blackshaw
      Pages 223-229

    3. High-Density Antigen Microarrays for the Assessment of Antibody Selectivity and Off-Target Binding

      • Ronald Sjöberg, Eni Andersson, Cecilia Hellström, Cecilia Mattsson, Jochen M. Schwenk, Peter Nilsson et al.
      Pages 231-238
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Keywords

About this book

This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field.




Editors and Affiliations

  • KTH School of Engineering Sciences in Chemistry, Biotechnology and Health, Protein Technology, Stockholm, Sweden

    Johan Rockberg

  • Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada

    Johan Nilvebrant

Bibliographic Information

  • Book Title: Epitope Mapping Protocols

  • Editors: Johan Rockberg, Johan Nilvebrant

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7841-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7839-7Published: 01 May 2018

  • Softcover ISBN: 978-1-4939-9301-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7841-0Published: 30 April 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 284

  • Number of Illustrations: 11 b/w illustrations, 38 illustrations in colour

  • Topics: Immunology

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