Digital PCR

1. Front Matter

   Pages i-xv

   PDF

2. Background Concepts

   1. Front Matter

      Pages 1-1

      PDF

   2. Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR

      • George Karlin-Neumann, Francisco Bizouarn

      Pages 3-10

   3. Basic Concepts and Validation of Digital PCR Measurements

      • Leonardo Pinheiro, Kerry R. Emslie

      Pages 11-24

   4. Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies–What Does It Mean?

      • Svilen Tzonev

      Pages 25-43

   5. Control Materials and Digital PCR Methods for Evaluation of Circulating Cell-Free DNA Extractions from Plasma

      • Alexandra S. Whale, Ana Fernandez-Gonzalez, Alice Gutteridge, Alison S. Devonshire

      Pages 45-65

3. Absolute Quantification

   1. Front Matter

      Pages 67-67

      PDF

   2. Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events

      • David Dobnik, Bjørn Spilsberg, Alexandra Bogožalec Košir, Dejan Štebih, Dany Morisset, Arne Holst-Jensen et al.

      Pages 69-98

   3. Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples

      • Ashley Vellucci, Emily C. Leibovitch, Steven Jacobson

      Pages 99-109

   4. Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR

      • Petar Podlesniy, Ramon Trullas

      Pages 111-126

   5. Testing of General and Human-Associated Fecal Contamination in Waters

      • Yiping Cao, Meredith R. Raith, John F. Griffith

      Pages 127-140

4. Copy Number Variation

   1. Front Matter

      Pages 141-141

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   2. Analyzing Copy Number Variation with Droplet Digital PCR

      • Avery Davis Bell, Christina L. Usher, Steven A. McCarroll

      Pages 143-160

   3. Assessing HER2 Amplification in Plasma cfDNA

      • Isaac Garcia-Murillas, Nicholas C. Turner

      Pages 161-172

   4. Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues Using ddPCR: Analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants

      • Bo Zhou, Michael S. Haney, Xiaowei Zhu, Reenal Pattni, Alexej Abyzov, Alexander E. Urban

      Pages 173-190

5. Rare Mutation and Rare Allele Detection

   1. Front Matter

      Pages 191-191

      PDF

   2. Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer Using Droplet Digital PCR

      • Yanan Kuang, Allison O’Connell, Adrian G. Sacher, Nora Feeney, Ryan S. Alden, Geoffrey R. Oxnard et al.

      Pages 193-207

   3. Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients

      • Arielle J. Medford, Riaz N. Gillani, Ben Ho Park

      Pages 209-227

   4. Droplet Digital PCR for Minimal Residual Disease Detection in Mature Lymphoproliferative Disorders

      • Daniela Drandi, Simone Ferrero, Marco Ladetto

      Pages 229-256

   5. Quantitation of JAK2 V617F Allele Burden by Using the QuantStudio™ 3D Digital PCR System

      • Elena Kinz, Axel Muendlein

      Pages 257-273

This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.”

• polymerase chain reaction
• droplet-based digital PCR
• infectious disease
• phenotypes
• somatic mosaicism
• genome editing
• cell therapy
• haplotyping

“The editors of the volume have succeeded in providing specialists in different sectors with a wide range of technology applications that can be adapted to other platforms or can provide the basis for creating new ones.” (Paolo Catarsi, European Journal of Histochemistry, Vol. 63, 2019)

• Digital Biology Center, Bio-Rad Laboratories, Pleasanton, USA

  George Karlin-Neumann, Francisco Bizouarn

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