Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols

1. Front Matter

   Pages i-xi

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2. Orchid Seeds in Plant Propagation and Conservation

   1. Front Matter

      Pages 1-1

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   2. Understanding Seed and Protocorm Development in Orchids

      • Edward C. Yeung, Yuan-Yuan Li, Yung-I Lee

      Pages 3-26

   3. Orchid Mycorrhizal Fungi: Isolation and Identification Techniques

      • Lawrence W. Zettler, Laura L. Corey

      Pages 27-59

   4. Protocols for Establishing Fungi-Protocorm Culture

      • Yuan-Yuan Li, Hui Wang, Shun-Xing Guo

      Pages 61-69

   5. Orchid Seed and Pollen: A Toolkit for Long-Term Storage, Viability Assessment and Conservation

      • Philip T. Seaton, Silvério T. Hosomi, Ceci C. Custódio, Timothy R. Marks, Nelson B. Machado-Neto, Hugh W. Pritchard

      Pages 71-98

3. Orchid Micropropagation

   1. Front Matter

      Pages 99-99

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   2. Orchid Seed Germination and Micropropagation I: Background Information and Related Protocols

      • Edward C. Yeung, Jungmin Park, Indra S. Harry

      Pages 101-125

   3. Orchid Seed Germination and Micropropagation II: Media Information and Composition

      • Jungmin Park, Edward C. Yeung

      Pages 127-150

   4. Orchid Micropropagation: An Overview of Approaches and Methodologies

      • Tim W. Yam, J. Arditti

      Pages 151-178

   5. Common Protocols in Orchid Micropropagation

      • So-Young Park, Yoon-Sun Huh, Kee-Yoeup Paek

      Pages 179-193

   6. Micropropagation of Orchids by Using Bioreactor Technology

      • Hosakatte Niranjana Murthy, Kee-Yoeup Paek, So-Young Park

      Pages 195-208

   7. Enhanced Growth and Development of Cymbidium and Phalaenopsis Plantlets Cultured In Vitro Under Light-Emitting Diodes

      • Duong Tan Nhut, Hoang Thanh Tung, Michio Tanaka

      Pages 209-223

4. Special Techniques

   1. Front Matter

      Pages 225-225

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   2. Tracking Pollen Fates in Orchid Populations

      • Steven D. Johnson, Lawrence D. Harder

      Pages 227-239

   3. Cut-Column Pollination Method to Overcome Pollination Barrier in Phalaenopsis

      • Hua-Ting Chuang, Kuang-Liang Huang, Shan-Te Hsu

      Pages 241-256

   4. In Vitro Flowering and Breeding of Erycina pusilla

      • Yi-Tien Chiu, Chen Chang

      Pages 257-265

   5. Virus Elimination Through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System

      • Rong-Show Shen, Shan-Te Hsu

      Pages 267-282

   6. Cryopreservation

      • Wagner A. Vendrame

      Pages 283-302

   7. Haploid Seed Formation via Parthenogenesis in Bletilla

      • Juntaro Kato, Syoichi Ichihashi

      Pages 303-315

The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation.  The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.

• Orchid seed biology
• conservation
• orchid micropropagation
• orchid transformation
• greenhouse techniques
• Systematic Botany

• Biology Department, National Museum of Natural Science, Taichung, China

  Yung-I Lee

• Department of Biological Sciences, University of Calgary, Calgary, Canada

  Edward Chee-Tak Yeung

Yung-I Lee received his Ph.D. degree in Horticultural Science from the National Taiwan University. After spending two years as a postdoctoral fellow at the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan, Dr. Lee joined the Department of Biology, National Museum of Natural Science, where he is now a Curator. His primary research interests have been the conservation biology of orchids, especially in vitro propagation and mycorrhizal association. Dr. Lee also served as the chairman of Orchid Working Group (2006-2010) in the International Society for Horticultural Science.


Edward Chee-Tak Yeung received his B. Sc. Honors degree from the University of Guelph and a Ph.D. in Biology from Yale University.  After spending one year as a postdoctoral fellow at the University of Ottawa, Dr. Yeung joined the Department of Biological Sciences, University of Calgary, Canada, where he is now a Faculty Professor. His primary research interests involvethe reproductive biology of higher plants, especially the structural and physiological aspects of embryo development. Professor Yeung is the recipient of the Lawson Medal in recognition of cumulative, life-time contributions to Canadian botany from the Canadian Botanical Association in 2014.  He has co-edited two books with colleagues, entitled “Plant Embryo Culture” and “Plant Microtechniques and Protocols”. Both are published by Springer Nature, New York.

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