Biomaterials for Tissue Engineering

1. Front Matter

   Pages i-xiv

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2. Engineering Citric Acid-Based Porous Scaffolds for Bone Regeneration

   • Jacqueline J. Masehi-Lano, Eun Ji Chung

   Pages 1-10

3. Multifunctional Self-Assembling Peptide-Based Nanostructures for Targeted Intracellular Delivery: Design, Physicochemical Characterization, and Biological Assessment

   • Yejiao Shi, Ran Lin, Honggang Cui, Helena S. Azevedo

   Pages 11-26

4. Electrospinning Functionalized Polymers for Use as Tissue Engineering Scaffolds

   • Lesley W. Chow

   Pages 27-39

5. Low-Temperature Deposition Modeling of β-TCP Scaffolds with Controlled Bimodal Porosity

   • E. Papastavrou, P. Breedon, D. Fairhurst

   Pages 41-54

6. Three-Dimensional Hydrogel-Based Culture to Study the Effects of Toxicants on Ovarian Follicles

   • Hong Zhou, Ariella Shikanov

   Pages 55-72

7. Layer-by-Layer Engineered Polymer Capsules for Therapeutic Delivery

   • Rona Chandrawati

   Pages 73-84

8. Controlling Fibrin Network Morphology, Polymerization, and Degradation Dynamics in Fibrin Gels for Promoting Tissue Repair

   • Erin P. Sproul, Riley T. Hannan, Ashley C. Brown

   Pages 85-99

9. Biofunctionalization of Poly(acrylamide) Gels

   • Julieta I. Paez, Aleeza Farrukh, Oya Ustahüseyin, Aránzazu del Campo

   Pages 101-114

10. Synthetic PEG Hydrogel for Engineering the Environment of Ovarian Follicles

    • Uziel Mendez, Hong Zhou, Ariella Shikanov

    Pages 115-128

11. Engineering Human Neural Tissue by 3D Bioprinting

    • Qi Gu, Eva Tomaskovic-Crook, Gordon G. Wallace, Jeremy M. Crook

    Pages 129-138

12. High-Throughput Formation of Mesenchymal Stem Cell Spheroids and Entrapment in Alginate Hydrogels

    • Charlotte E. Vorwald, Steve S. Ho, Jacklyn Whitehead, J. Kent Leach

    Pages 139-149

13. Crimped Electrospun Fibers for Tissue Engineering

    • Pen-hsiu Grace Chao

    Pages 151-159

14. In Vitro Model of Macrophage-Biomaterial Interactions

    • Claire E. Witherel, Pamela L. Graney, Kara L. Spiller

    Pages 161-176

15. Synthesis of Self-Assembling Peptide-Based Hydrogels for Regenerative Medicine Using Solid-Phase Peptide Synthesis

    • E. Thomas Pashuck

    Pages 177-192

16. H2S Delivery from Aromatic Peptide Amphiphile Hydrogels

    • Kuljeet Kaur, Yun Qian, John B. Matson

    Pages 193-208

17. Back Matter

    Pages 209-212

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This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study.

• tissue engineering
• regenerative medicine
• biomaterials
• drug delivery
• hydrogel

• Cellerant Therapeutics, San Carlos, USA

  Kanika Chawla

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