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Decellularized Scaffolds and Organogenesis

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Hospital Research Institute, Ottawa, Canada

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  • Includes cutting-edge methods and protocols for the study of decellularization and organogenesis
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1577)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Decellularization Methods for Scaffold Fabrication

    • Sweta K. Gupta, Narayan C. Mishra, Archna Dhasmana
    Pages 1-10

  3. Decellularized Liver Scaffold for Liver Regeneration

    • Wei Yang, Renpei Xia, Yujun Zhang, Hongyu Zhang, Lianhua Bai
    Pages 11-23

  4. Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications

    • Gabriel L. Converse, Dandan Li, Eric E. Buse, Richard A. Hopkins, Omar S. Aljitawi
    Pages 25-33

  5. 3D Decellularized Native Extracellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells (hWJ MSCs)

    • Balasubramanian Sundaram, Anne George Cherian, Sanjay Kumar
    Pages 35-53

  6. Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells

    • Clemens Gögele, Silke Schwarz, Benjamin Ondruschka, Niels Hammer, Gundula Schulze-Tanzil
    Pages 55-69

  7. Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells

    • Takashi Hoshiba, Naoki Kawazoe, Guoping Chen
    Pages 71-86

  8. Mouse Skeletal Muscle Decellularization

    • Martina Piccoli, Caterina Trevisan, Edoardo Maghin, Chiara Franzin, Michela Pozzobon
    Pages 87-93

  9. Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering

    • Matthew J. Hodgson, Christopher C. Knutson, Nima Momtahan, Alonzo D. Cook
    Pages 95-102

  10. In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering

    • Andrea Peloso, Antonio Citro, Valeria Corradetti, Szandra Brambilla, Graziano Oldani, Francesco Calabrese et al.
    Pages 103-110

  11. Glutaraldehyde Cross-linking Modification of Decellularized Rat Kidney Scaffolds

    • Yaling Yu, Li Liu, Jianse Zhang, Zairong Wei, Jin Mei
    Pages 111-119

  12. Preparation of Scaffolds from Decellularized Testicular Matrix

    • Yoni Baert, Ellen Goossens
    Pages 121-127

  13. Decellularization of Bovine Small Intestinal Submucosa

    • Mahmut Parmaksiz, AyÅŸe Eser Elçin, YaÅŸar Murat Elçin
    Pages 129-138

  14. Decellularization and Recellularization of Cartilage

    • Catherine A. Bautista, Bahar Bilgen
    Pages 139-146

  15. A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

    • Jingting Li, Ming Pei
    Pages 147-154

  16. Tracheal Cartilage Isolation and Decellularization

    • Zachary Galliger, Angela Panoskaltsis-Mortari
    Pages 155-160

  17. Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing

    • Arvind Manikantan Padma, Tom Tristan Tiemann, Ahmed Baker Alshaikh, Randa Akouri, Min Jong Song, Mats Hellström
    Pages 161-175

  18. Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation

    • Enes Taylan, Kutluk Oktay
    Pages 177-181

  19. Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component Within Hydrogels as In Vitro Models and Cell Delivery Systems

    • Arthi Shridhar, Elizabeth Gillies, Brian G. Amsden, Lauren E. Flynn
    Pages 183-208

  20. 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

    • Alexandra N. Rindone, Ethan Nyberg, Warren L. Grayson
    Pages 209-226
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Keywords

About this book

This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Decellularized Scaffolds and Organogenesis: Methods and Protocols share novel approaches and insights that will provide new opportunities for those already in the field or moving to enter the field.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Decellularized Scaffolds and Organogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7656-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7655-3Published: 28 November 2018

  • eBook ISBN: 978-1-4939-7656-0Published: 28 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 347

  • Number of Illustrations: 16 b/w illustrations, 65 illustrations in colour

  • Topics: Cell Biology

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