Decellularized Scaffolds and Organogenesis

1. Front Matter

   Pages i-xvi

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2. Decellularization Methods for Scaffold Fabrication

   • Sweta K. Gupta, Narayan C. Mishra, Archna Dhasmana

   Pages 1-10

3. Decellularized Liver Scaffold for Liver Regeneration

   • Wei Yang, Renpei Xia, Yujun Zhang, Hongyu Zhang, Lianhua Bai

   Pages 11-23

4. Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications

   • Gabriel L. Converse, Dandan Li, Eric E. Buse, Richard A. Hopkins, Omar S. Aljitawi

   Pages 25-33

5. 3D Decellularized Native Extracellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells (hWJ MSCs)

   • Balasubramanian Sundaram, Anne George Cherian, Sanjay Kumar

   Pages 35-53

6. Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells

   • Clemens Gögele, Silke Schwarz, Benjamin Ondruschka, Niels Hammer, Gundula Schulze-Tanzil

   Pages 55-69

7. Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells

   • Takashi Hoshiba, Naoki Kawazoe, Guoping Chen

   Pages 71-86

8. Mouse Skeletal Muscle Decellularization

   • Martina Piccoli, Caterina Trevisan, Edoardo Maghin, Chiara Franzin, Michela Pozzobon

   Pages 87-93

9. Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering

   • Matthew J. Hodgson, Christopher C. Knutson, Nima Momtahan, Alonzo D. Cook

   Pages 95-102

10. In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering

    • Andrea Peloso, Antonio Citro, Valeria Corradetti, Szandra Brambilla, Graziano Oldani, Francesco Calabrese et al.

    Pages 103-110

11. Glutaraldehyde Cross-linking Modification of Decellularized Rat Kidney Scaffolds

    • Yaling Yu, Li Liu, Jianse Zhang, Zairong Wei, Jin Mei

    Pages 111-119

12. Preparation of Scaffolds from Decellularized Testicular Matrix

    • Yoni Baert, Ellen Goossens

    Pages 121-127

13. Decellularization of Bovine Small Intestinal Submucosa

    • Mahmut Parmaksiz, Ayşe Eser Elçin, Yaşar Murat Elçin

    Pages 129-138

14. Decellularization and Recellularization of Cartilage

    • Catherine A. Bautista, Bahar Bilgen

    Pages 139-146

15. A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

    • Jingting Li, Ming Pei

    Pages 147-154

16. Tracheal Cartilage Isolation and Decellularization

    • Zachary Galliger, Angela Panoskaltsis-Mortari

    Pages 155-160

17. Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing

    • Arvind Manikantan Padma, Tom Tristan Tiemann, Ahmed Baker Alshaikh, Randa Akouri, Min Jong Song, Mats Hellström

    Pages 161-175

18. Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation

    • Enes Taylan, Kutluk Oktay

    Pages 177-181

19. Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component Within Hydrogels as In Vitro Models and Cell Delivery Systems

    • Arthi Shridhar, Elizabeth Gillies, Brian G. Amsden, Lauren E. Flynn

    Pages 183-208

20. 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

    • Alexandra N. Rindone, Ethan Nyberg, Warren L. Grayson

    Pages 209-226

This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Decellularized Scaffolds and Organogenesis: Methods and Protocols share novel approaches and insights that will provide new opportunities for those already in the field or moving to enter the field.

• Regenerative medicine
• Bioscaffolds
• Decellularizing tissues
• Organ formation
• Bioengineering

• Ottawa Hospital Research Institute, Ottawa, Canada

  Kursad Turksen

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