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Organoids

Stem Cells, Structure, and Function

  • Book
  • © 2019

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Hospital Research Institute, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of organoids
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1576)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment

    • Magdalena Sutcliffe, Madeline A. Lancaster
    Pages 1-12

  3. Clinically Amendable, Defined, and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells

    • Eva Tomaskovic-Crook, Jeremy M. Crook
    Pages 13-22

  4. Organoid Culture of Human Cancer Stem Cells

    • Yohei Shimono, Junko Mukohyama, Taichi Isobe, Darius M. Johnston, Piero Dalerba, Akira Suzuki
    Pages 23-31

  5. Construction of Thymus Organoids from Decellularized Thymus Scaffolds

    • Asako Tajima, Isha Pradhan, Xuehui Geng, Massimo Trucco, Yong Fan
    Pages 33-42

  6. Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres

    • Robert E. Hynds, Colin R. Butler, Sam M. Janes, Adam Giangreco
    Pages 43-53

  7. Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells

    • Mahboobe Ghaedi, Laura E. Niklason
    Pages 55-92

  8. Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix

    • Hiroko Hisha, Hiroo Ueno
    Pages 93-99

  9. Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension

    • Valentina Benedetti, Valerio Brizi, Christodoulos Xinaris
    Pages 101-112

  10. Efficient Culture of Intestinal Organoids with Blebbistatin

    • Zhen Qi, Ye-Guang Chen
    Pages 113-121

  11. Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-recombinase-Mediated Gene Deletion

    • Dustin J. Flanagan, Renate H. M. Schwab, Bang M. Tran, Toby J. Phesse, Elizabeth Vincan
    Pages 123-133

  12. The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine

    • Malgorzata Pierzchalska, Malgorzata Panek, Malgorzata Czyrnek, Maja Grabacka
    Pages 135-144

  13. New Trends and Perspectives in the Function of Non-neuronal Acetylcholine in Crypt–Villus Organoids in Mice

    • Toshio Takahashi
    Pages 145-155

  14. Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System

    • Jessica L. Forbester, Nicholas Hannan, Ludovic Vallier, Gordon Dougan
    Pages 157-169

  15. Murine Colonic Organoid Culture System and Downstream Assay Applications

    • Yang-Yi Fan, Laurie A. Davidson, Robert S. Chapkin
    Pages 171-181

  16. Intestinal Organoids as a Novel Tool to Study Microbes–Epithelium Interactions

    • Giulia Nigro, Melissa Hanson, Cindy Fevre, Marc Lecuit, Philippe J. Sansonetti
    Pages 183-194

  17. The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism

    • Diana Li, Hongli Dong, Alison B. Kohan
    Pages 195-204

  18. Oncogenic Transformation of Human-Derived Gastric Organoids

    • Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, Yana Zavros
    Pages 205-213

  19. Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation

    • Yun Chen, Chuan Li, Ya-Hui Tsai, Sheng-Hong Tseng
    Pages 215-228

  20. Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections

    • Winnie Y. Zou, Sarah E. Blutt, Sue E. Crawford, Khalil Ettayebi, Xi-Lei Zeng, Kapil Saxena et al.
    Pages 229-247
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Keywords

About this book

This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Organoids

  • Book Subtitle: Stem Cells, Structure, and Function

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7617-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-7616-4Published: 13 September 2019

  • eBook ISBN: 978-1-4939-7617-1Published: 12 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 363

  • Number of Illustrations: 23 b/w illustrations, 49 illustrations in colour

  • Topics: Cell Biology

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