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Host-Pathogen Interactions

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Carlos Medina

    1. Departamento de Biología Molecular e Ingeniería Bioquímica, Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de, Olavide/Junta de Andalucía, Seville, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Francisco Javier López-Baena

    1. Department of Microbiology, University of Seville, Sevilla, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of host-pathogen interactions in animals and plants
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1734)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Genetic Association Studies in Host–Pathogen Interaction Analysis

    • Jose Luis Royo, Luis Miguel Real
    Pages 1-11

  3. Bacterial Genotyping Methods: From the Basics to Modern

    • Margarita M. Ochoa-Díaz, Silvana Daza-Giovannetty, Doris Gómez-Camargo
    Pages 13-20

  4. Animal-Pathogen Interaction Section

    1. Front Matter

      Pages 21-21
      Download chapter PDF

    2. Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

      • Gili Aviv, Ohad Gal-Mor
      Pages 23-32

    3. Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection

      • Gili Aviv, Ohad Gal-Mor
      Pages 33-38

    4. lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

      • Gili Aviv, Ohad Gal-Mor
      Pages 39-45

    5. Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity

      • Gili Aviv, Ohad Gal-Mor
      Pages 47-54

    6. Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage

      • Carlos Medina, Beatriz Mesa-Pereira, Eva M. Camacho, Amando Flores, Eduardo Santero
      Pages 55-70

    7. Organoids as a Model to Study Infectious Disease

      • Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, Eitaro Aihara
      Pages 71-81

    8. Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins

      • Anita Horvatić, Josipa Kuleš, Nicolas Guillemin, Franjo Martinković, Iva Štimac, Vladimir Mrljak et al.
      Pages 83-96

    9. Systems Biology Modeling to Study Pathogen–Host Interactions

      • Müberra Fatma Cesur, Saliha Durmuş
      Pages 97-112

    10. Phage Therapy: Various Perspectives on How to Improve the Art

      • Stephen T. Abedon
      Pages 113-127

  6. Plant-Pathogen Interaction Section

    1. Front Matter

      Pages 181-181
      Download chapter PDF

    2. Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae

      • José S. Rufián, Diego López-Márquez, Nieves López-Pagán, Murray Grant, Javier Ruiz-Albert, Carmen R. Beuzón
      Pages 183-199

    3. Introduction of Genetic Material in Ralstonia solanacearum Through Natural Transformation and Conjugation

      • Anthony Perrier, Patrick Barberis, Stéphane Genin
      Pages 201-207
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Keywords

About this book

This detailed volume explores numerous multidisciplinary approaches employed to analyze the role of different molecules or strategies used by different guests to survive inside hosts. Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Host-Pathogen Interactions: Methods and Protocols provides many molecular and bioinformatics techniques currently in use at reputed laboratories in countries all around the world, vital for many kinds of host-pathogen interactions studies.

Editors and Affiliations

  • Departamento de Biología Molecular e Ingeniería Bioquímica, Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de, Olavide/Junta de Andalucía, Seville, Spain

    Carlos Medina

  • Department of Microbiology, University of Seville, Sevilla, Spain

    Francisco Javier López-Baena

Bibliographic Information

  • Book Title: Host-Pathogen Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Carlos Medina, Francisco Javier López-Baena

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7604-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7603-4Published: 30 December 2017

  • Softcover ISBN: 978-1-4939-8532-6Published: 23 May 2018

  • eBook ISBN: 978-1-4939-7604-1Published: 28 December 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 317

  • Number of Illustrations: 12 b/w illustrations, 53 illustrations in colour

  • Topics: Microbiology

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