Bacterial Chemosensing

1. Front Matter

   Pages i-xiii

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2. Introduction

   1. Front Matter

      Pages 1-1

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   2. The Diversity of Bacterial Chemosensing

      • Michael D. Manson

      Pages 3-6

   3. Transmembrane Signal Transduction in Bacterial Chemosensing

      • Michael D. Manson

      Pages 7-19

3. Methods for Studying Bacterial Chemotaxis at the Population and Whole-Cell Levels

   1. Front Matter

      Pages 21-21

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   2. Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay

      • Victoria Korolik, Karen M. Ottemann

      Pages 23-31

   3. Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries

      • Benjamin A. Webb, Timofey D. Arapov, Birgit E. Scharf

      Pages 33-45

   4. A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradients

      • Nitesh Sule, Daniel Penarete-Acosta, Derek L. Englert, Arul Jayaraman

      Pages 47-59

   5. Visualizing Chemoattraction of Planktonic Cells to a Biofilm

      • Sneha Jani

      Pages 61-69

   6. Labeling Bacterial Flagella with Fluorescent Dyes

      • Linda Turner, Howard C. Berg

      Pages 71-76

4. In Vivo Analysis of Receptor Function

   1. Front Matter

      Pages 77-77

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   2. All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins

      • Peter Ames, John S. Parkinson

      Pages 79-85

   3. Mutational Analysis of Binding Protein–Chemoreceptor Interactions

      • Andrew L. Seely

      Pages 87-94

   4. In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway

      • Hanna E. Walukiewicz, George W. Ordal, Christopher V. Rao

      Pages 95-105

   5. FRET Analysis of the Chemotaxis Pathway Response

      • Anja Paulick, Victor Sourjik

      Pages 107-126

   6. Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout

      • Run-Zhi Lai, John S. Parkinson

      Pages 127-135

   7. Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation

      • Kylie J. Watts, Mark S. Johnson

      Pages 137-145

   8. Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid–Aqueous Interface

      • Rahmi Yusuf, Robert J. Lawrence, Lucy V. Eke, Roger R. Draheim

      Pages 147-158

   9. Analyzing Chemoreceptor Interactions In Vivo with the Trifunctional Cross-Linker TMEA

      • Claudia A. Studdert, Diego A. Massazza

      Pages 159-170

5. Cryo-EM Methods for Studying Chemoreceptor Structure

   1. Front Matter

      Pages 171-171

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This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world.

• Chemoreceptor
• Chemotaxis
• Chemosensory behavior
• Signal transduction system
• Cryo-electron tomography
• Intracellular movement

• Department of Biology, Texas A&M University, College Station, USA

  Michael D. Manson

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