The Surfaceome

1. Front Matter

   Pages i-xi

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2. Discovery-Based Approaches to Surfaceome Content

   1. Front Matter

      Pages 1-1

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   2. Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens

      • Yulanda M. Williamson, Jennifer Whitmon, Rolieria West-Deadwyler, Hercules Moura, Adrian R. Woolfitt, Jon Rees et al.

      Pages 3-20

   3. “Shaving” Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins

      • Manuel J. Rodríguez-Ortega

      Pages 21-29

   4. Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes

      • Witold W. Kilarski, John Herbert, Andreas Bikfalvi

      Pages 31-56

   5. Mass Spectrometry-Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and Their Derivatives

      • Chelsea M. Fujinaka, Matthew Waas, Rebekah L. Gundry

      Pages 57-78

   6. Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and Its Proteome from Cells in Suspension

      • Rebecca L. Rose, Waeowalee Choksawangkarn, Catherine Fenselau

      Pages 79-90

   7. Proteomic Profiling of Secreted Proteins, Exosomes, and Microvesicles in Cell Culture Conditioned Media

      • Ankit Sinha, Simona Principe, Javier Alfaro, Alex Ignatchenko, Vladimir Ignatchenko, Thomas Kislinger

      Pages 91-102

3. Targeted Approaches for Surfaceome Content

   1. Front Matter

      Pages 103-103

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   2. Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-Phosphate Receptor, from Sf9 Cells Using the Baculovirus System

      • Linda J. Olson, Nancy M. Dahms

      Pages 105-116

   3. Bispecific Antibody Armed T Cells to Target Cancer Cells

      • Archana Thakur, Lawrence G. Lum, Sandeep Mittal

      Pages 117-126

   4. Immunophenotyping of Live Human Pluripotent Stem Cells by Flow Cytometry

      • Daniel R. Riordon, Kenneth R. Boheler

      Pages 127-149

   5. Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4

      • Amanda M. Nevins, Adriano Marchese

      Pages 151-164

4. Cell-Based Functional Analyses Related to Surfaceome Content

   1. Front Matter

      Pages 165-165

      PDF

   2. NaV Channels: Assaying Biosynthesis, Trafficking, Function

      • Gordon F. Tomaselli, Federica Farinelli

      Pages 167-184

   3. High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs)

      • Chi-Wing Kong, Lin Geng, Ronald A. Li

      Pages 185-194

   4. Methods for Evaluation of Vascular Endothelial Cell Function with Transient Receptor Potential (TRP) Channel Drugs

      • Yung Wui Tjong, Xiaoqiang Yao

      Pages 195-210

   5. Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase TrkB in Neurons

      • Kwok-On Lai, Nancy Y. Ip

      Pages 211-222

   6. Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes

      • Vladimir Khristov, Qin Wan, Ruchi Sharma, Mostafa Lotfi, Arvydas Maminishkis, Kapil Bharti

      Pages 223-247

   7. Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells Under Flow

      • Teresa Massam-Wu, Stuart A. Cain, Cay M. Kielty

      Pages 249-260

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions totheir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.

• plasma membrane
• cell surface proteins
• antibodies
• lipids and glycans
• immunophenotyping markers
• avian embryos and mammalian systems
• Cell Surface Capture Technology
• n-glycoproteins
• G protein couple receptors CXCR4
• fluid transport
• Targets-search
• bioinformatics

• School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

  Kenneth R. Boheler

• Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA

  Rebekah L. Gundry

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