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Innate Immune Activation

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1714)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
  2. Emerging Concepts in Innate Immunity

    • Karin Pelka, Dominic De Nardo
    Pages 1-18
  3. Bioinformatic Assessment of Macrophage Activation by the Innate Immune System

    • Thomas Ulas, Joachim L. Schultze, Marc Beyer
    Pages 19-40
  4. Modeling Primary Human Monocytes with the Trans–Differentiation Cell Line BLaER1

    • Moritz M. Gaidt, Francesca Rapino, Thomas Graf, Veit Hornung
    Pages 57-66
  5. Measurement of NF-κB Activation in TLR-Activated Macrophages

    • Orna Ernst, Sharat J. Vayttaden, Iain D. C. Fraser
    Pages 67-78
  6. Biochemical Isolation of the Myddosome from Murine Macrophages

    • Yunhao Tan, Jonathan C. Kagan
    Pages 79-95
  7. Generation of Innate Immune Reporter Cells Using Retroviral Transduction

    • Yamel Cardona Gloria, Eicke Latz, Dominic De Nardo
    Pages 97-117
  8. Examining Myddosome Formation by Luminescence-Based Mammalian Interactome Mapping (LUMIER)

    • Olaf-Oliver Wolz, Manfred Koegl, Alexander N. R. Weber
    Pages 119-130
  9. Detection of ASC Speck Formation by Flow Cytometry and Chemical Cross-linking

    • Florian Hoss, Verena Rolfes, Mariana R. Davanso, Tarcio T. Braga, Bernardo S. Franklin
    Pages 149-165
  10. Measuring Innate Immune Responses to Bacterial Viability

    • Julien Moretti, Nicolas Vabret, J. Magarian Blander
    Pages 167-190
  11. Methods to Study Cell Swelling-Induced Inflammasome Activation

    • Vincent Compan, Pablo Pelegrín
    Pages 191-197
  12. Detecting Release of Bacterial dsDNA into the Host Cytosol Using Fluorescence Microscopy

    • Roland Felix Dreier, José Carlos Santos, Petr Broz
    Pages 199-213
  13. Quantitative Proteomics of Secreted Proteins

    • Annika Frauenstein, Felix Meissner
    Pages 215-227
  14. Simultaneous Detection of Cellular Viability and Interleukin-1β Secretion from Single Cells by ELISpot

    • Stephanie A. Conos, Lisa M. Lindqvist, James E. Vince
    Pages 229-236
  15. Detection and Quantification of MAVS Aggregation via Confocal Microscopy

    • Tan A. Nguyen, Lachlan Whitehead, Ken C. Pang
    Pages 237-247
  16. Back Matter

    Pages 249-251

About this book

This volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Innate Immune Activation: Methods and Protocols aims to be a useful and informative reference tool for further study into this vital field.

Editors and Affiliations

  • Inflammation Division, The Walter & Eliza Hall Institute of Medical Research, Parkville, Australia

    Dominic De Nardo

  • Chemical Biology Division, The Walter & Eliza Hall Institute of Medical Research, Parkville, Australia

    Christine M De Nardo

Bibliographic Information

  • Book Title: Innate Immune Activation

  • Book Subtitle: Methods and Protocols

  • Editors: Dominic De Nardo, Christine M De Nardo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7519-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7518-1Published: 25 November 2017

  • Softcover ISBN: 978-1-4939-8508-1Published: 05 September 2018

  • eBook ISBN: 978-1-4939-7519-8Published: 24 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 251

  • Number of Illustrations: 14 b/w illustrations, 40 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access