Methods in Molecular Biology

DNA Methylation Protocols

Editors: Tost, Jörg (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7481-8
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Hardcover $159.00
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About this book

This third edition volume expands on the previous editions by providing a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.

Table of contents (16 chapters)

  • Large-Scale Targeted DNA Methylation Analysis Using Bisulfite Padlock Probes

    Dinh Diep, Nongluk Plongthongkum, Kun Zhang

    Pages

  • Tagmentation-Based Library Preparation for Low DNA Input Whole Genome Bisulfite Sequencing

    Dieter Weichenhan, et al.

    Pages

  • Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)

    Fides D. Lay, Theresa K. Kelly, Peter A. Jones

    Pages

  • A Guide to Illumina BeadChip Data Analysis

    Michael C. Wu, Pei-Fen Kuan

    Pages

  • DNA Methylation Analysis of Free-Circulating DNA in Body Fluids

    Maria Jung, Glen Kristiansen, Dimo Dietrich

    Pages

Buy this book

eBook $119.00
price for USA (gross)
  • ISBN 978-1-4939-7481-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.00
price for USA
  • ISBN 978-1-4939-7479-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
DNA Methylation Protocols
Editors
  • Jörg Tost
Series Title
Methods in Molecular Biology
Series Volume
1708
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7481-8
DOI
10.1007/978-1-4939-7481-8
Hardcover ISBN
978-1-4939-7479-5
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XVIII, 704
Number of Illustrations and Tables
35 b/w illustrations, 67 illustrations in colour
Topics