Erythropoiesis

1. Front Matter

   Pages i-xi

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2. An Introduction to Erythropoiesis Approaches

   • Joyce A. Lloyd

   Pages 1-10

3. Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis

   • Martin D. Kafina, Barry H. Paw

   Pages 11-36

4. Mouse Models of Erythropoiesis and Associated Diseases

   • Matthew P. Parker, Kenneth R. Peterson

   Pages 37-65

5. Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells

   • Skye C. McIver, Kyle J. Hewitt, Xin Gao, Charu Mehta, Jing Zhang, Emery H. Bresnick

   Pages 67-89

6. Stress Erythropoiesis Model Systems

   • Laura F. Bennett, Chang Liao, Robert F. Paulson

   Pages 91-102

7. Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models

   • Marie Trudel, Josepha-Clara Sedzro

   Pages 103-115

8. Functional Analysis of Erythroid Progenitors by Colony-Forming Assays

   • James Palis, Anne Koniski

   Pages 117-132

9. Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands

   • Jia Hao Yeo, Matthew P. Cosgriff, Stuart T. Fraser

   Pages 133-152

10. Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells

    • Xiuli An, Lixiang Chen

    Pages 153-174

11. Analysis of Erythropoiesis Using Imaging Flow Cytometry

    • Theodosia Kalfa, Kathleen E. McGrath

    Pages 175-192

12. Flow Cytometric Analysis of Erythroblast Enucleation

    • Xiuli An, Lixiang Chen

    Pages 193-203

13. High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure

    • Alyson S. Smith, Roberta B. Nowak, Velia M. Fowler

    Pages 205-228

14. Chromatin Immunoprecipitation (ChIP) with Erythroid Samples

    • Ivan Krivega, Ann Dean

    Pages 229-236

15. Chromosome Conformation Capture (3C and Higher) with Erythroid Samples

    • Ivan Krivega, Ann Dean

    Pages 237-243

16. Genome Editing of Erythroid Cell Culture Model Systems

    • Jinfen J. Yik, Merlin Crossley, Kate G. R. Quinlan

    Pages 245-257

17. In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood

    • Anna Kovilakath, Safa Mohamad, Farrah Hermes, Shou Zhen Wang, Gordon D. Ginder, Joyce A. Lloyd

    Pages 259-274

18. Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines

    • Divya S. Vinjamur, Daniel E. Bauer

    Pages 275-284

19. Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products

    • Neil W. A. McGowan, John D. M. Campbell, Joanne C. Mountford

    Pages 285-292

20. Back Matter

    Pages 293-298

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This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Erythropoiesis: Methods and Protocols provides a definitive source for molecular and cellular biology protocols to study erythropoiesis and to move this vital field forward.

• Animal models
• Red blood cells
• Progenitor cell status
• Erythroid cells
• Chromatin immunoprecipitation (ChIP)
• Erythroblast structure
• Erythrocytes

• Department of Human & Molecular Genetics, Virginia Commonwealth University, Richmond, USA

  Joyce A. Lloyd

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