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Sphingosine-1-Phosphate

Methods and Protocols

  • Includes cutting-edge techniques for the study of sphingosine-1-phosphate
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1697)

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Softcover Book USD 139.99
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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
  2. An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity

    • Melissa R. Pitman, Lorena T. Davies, Stuart M. Pitson
    Pages 9-20
  3. Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells

    • Alena P. Chumanevich, Piper A. Wedman, Carole A. Oskeritzian
    Pages 21-30
  4. In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate

    • Melina G. Castro, Ludmila E. Campos, Yamila I. Rodriguez, Sergio E. Alvarez
    Pages 117-131
  5. Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors

    • Phillip Callihan, Mohammed Alqinyah, Shelley B. Hooks
    Pages 141-151
  6. Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation

    • Guanghu Wang, Stefka D. Spassieva, Erhard Bieberich
    Pages 153-171
  7. 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering

    • Shrestha Priyadarsini, Sarah E. Nicholas, Dimitrios Karamichos
    Pages 173-180
  8. Back Matter

    Pages 181-182

About this book

This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in developmentand in physiopathology.  As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field.

Editors and Affiliations

  • Centre for Eye Research Australia, The University of Melbourne, East Melbourne, Australia

    Alice Pébay

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Sphingosine-1-Phosphate

  • Book Subtitle: Methods and Protocols

  • Editors: Alice Pébay, Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7413-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7412-2Published: 03 December 2017

  • Softcover ISBN: 978-1-4939-8478-7Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7413-9Published: 01 December 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 182

  • Number of Illustrations: 26 b/w illustrations

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access