Skip to main content
  • Book
  • © 2018

The Plant Endoplasmic Reticulum

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1691)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Labeling the ER for Light and Fluorescence Microscopy

    • Chris Hawes, Pengwei Wang, Verena Kriechbaumer
    Pages 1-14
  3. 3D Electron Microscopy of the ER

    • Maike Kittelmann
    Pages 15-21
  4. Characterization of Proteins Localized to Plant ER-PM Contact Sites

    • Pengwei Wang, Chris Hawes, Christine Richardson, Patrick J. Hussey
    Pages 23-31
  5. Quantitation of ER Structure and Function

    • Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara, Stefanie J. Müller, Andreas J. Meyer
    Pages 43-66
  6. Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination

    • Natasha Dzimitrowicz, Emily Breeze, Lorenzo Frigerio
    Pages 67-74
  7. ER Microsome Preparation in Arabidopsis thaliana

    • Verena Kriechbaumer
    Pages 117-123
  8. ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis

    • Laetitia Fouillen, Lilly Maneta-Peyret, Patrick Moreau
    Pages 125-137
  9. 2in1 Vectors Improve In Planta BiFC and FRET Analyses

    • Dietmar G. Mehlhorn, Niklas Wallmeroth, Kenneth W. Berendzen, Christopher Grefen
    Pages 139-158
  10. Metabolons on the Plant ER

    • Verena Kriechbaumer, Stanley W. Botchway
    Pages 159-166
  11. Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells

    • Imogen Sparkes, Rhiannon R. White, Benjamin Coles, Stanley W. Botchway, Andy Ward
    Pages 167-178
  12. Protein Biosynthesis and Maturation in the ER

    • Emanuela Pedrazzini, Alessandro Vitale
    Pages 179-189
  13. ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)

    • Lisa Yasmin Asseck, Niklas Wallmeroth, Christopher Grefen
    Pages 191-203
  14. Analysis of Protein Glycosylation in the ER

    • Jennifer Schoberer, Yun-Ji Shin, Ulrike Vavra, Christiane Veit, Richard Strasser
    Pages 205-222
  15. The Unfolded Protein Response

    • Kazuki Tabara, Yuji Iwata, Nozomu Koizumi
    Pages 223-230
  16. Unfolded Protein Response in Arabidopsis

    • Cristina Ruberti, Federica Brandizzi
    Pages 231-238

About this book

This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field. 

Editors and Affiliations

  • Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Chris Hawes, Verena Kriechbaumer

Bibliographic Information

  • Book Title: The Plant Endoplasmic Reticulum

  • Book Subtitle: Methods and Protocols

  • Editors: Chris Hawes, Verena Kriechbaumer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7389-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7388-0Published: 20 October 2017

  • Softcover ISBN: 978-1-4939-8470-1Published: 23 August 2018

  • eBook ISBN: 978-1-4939-7389-7Published: 17 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 265

  • Number of Illustrations: 12 b/w illustrations, 51 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access