Skip to main content
SpringerLink
Log in
SpringerLink
Log in

Quorum Sensing

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Livia Leoni

    1. Department of Science, University Roma Tre, Rome, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Giordano Rampioni

    1. Department of Science, University Roma Tre, Rome, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of quorum sensing
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1673)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Detection and Quantification of Quorum Sensing Signal Molecules

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Use of Whole-Cell Bioassays for Screening Quorum Signaling, Quorum Interference, and Biofilm Dispersion

      • Starla G. Thornhill, Robert J. C. McLean
      Pages 3-24

    3. Detection of 2-Alkyl-4-Quinolones Using Biosensors

      • Matthew P. Fletcher, Stephen P. Diggle, Miguel Cámara, Paul Williams
      Pages 25-34

    4. “Hot Stuff”: The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum-Sensing Signals

      • Amy L. Schaefer, Caroline S. Harwood, E. Peter Greenberg
      Pages 35-47

    5. Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)

      • François Lépine, Sylvain Milot, Marie-Christine Groleau, Eric Déziel
      Pages 49-59

    6. Detection of the Bacterial Quorum-Sensing Signaling Molecules N-Acyl-Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh-Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)

      • Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin, Anton Hartmann
      Pages 61-72

    7. Biosensors for the Detection and Quantification of AI-2 Class Quorum-Sensing Compounds

      • Sathish Rajamani, Richard Sayre
      Pages 73-88

    8. Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus

      • Ewan J. Murray, Paul Williams
      Pages 89-96

    9. Ultra-Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF) Family Quorum-Sensing Signals

      • Lian Zhou, Shuang Sun, Wei Zhang, Ya-Wen He
      Pages 97-105

    10. Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode

      • Alyah Buzid, John H. T. Luong, F. Jerry Reen, Fergal O’Gara, Jeremy D. Glennon, Gerard P. McGlacken
      Pages 107-116

    11. Detection and Quantification of Butyrolactones from Streptomyces

      • Marc Biarnes-Carrera, Rainer Breitling, Eriko Takano
      Pages 117-128

  3. Methods for the Studying of Quorum Sensing at the Molecular, Physiological, and Population Level

    1. Front Matter

      Pages 129-129
      Download chapter PDF

    2. Fluorescence Quenching Studies of γ-Butyrolactone-Binding Protein (CprB) from Streptomyces coelicolor A3(2)

      • Jessy Mariam, Ruchi Anand
      Pages 131-143

    3. Methods to Study Solo/Orphan Quorum-Sensing Receptors

      • Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, Brian M. M. Ahmer
      Pages 145-159

    4. Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases

      • Daniel Shin, Rajesh Nagarajan
      Pages 161-176

    5. Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq

      • Charlotte D. Majerczyk
      Pages 177-192

    6. Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics

      • Yilei Liu, Gabriella Pessi, Katharina Riedel, Leo Eberl
      Pages 193-202

    7. Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

      • Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt
      Pages 203-212

    8. Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells

      • Jake Everett, Rebecca Gabrilska, Kendra P. Rumbaugh, Elena Vikström
      Pages 213-225
Back to top

Keywords

About this book

This detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and quantification of quorum sensing signal molecules, methods for the studying of quorum sensing at the molecular, physiological, and population level, as well as identification and characterization of anti-quorum sensing agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Quorum Sensing: Methods and Protocols serves as a comprehensive guide to the most important methodology currently available in the field.

Editors and Affiliations

  • Department of Science, University Roma Tre, Rome, Italy

    Livia Leoni, Giordano Rampioni

Bibliographic Information

  • Book Title: Quorum Sensing

  • Book Subtitle: Methods and Protocols

  • Editors: Livia Leoni, Giordano Rampioni

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7309-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7308-8Published: 15 November 2017

  • Softcover ISBN: 978-1-4939-8448-0Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7309-5Published: 11 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 375

  • Number of Illustrations: 45 b/w illustrations, 49 illustrations in colour

  • Topics: Microbiology

Publish with us

Policies and ethics

Back to top

Search

Navigation