Methods in Molecular Biology

Innate Antiviral Immunity

Methods and Protocols

Editors: Mossman, Karen (Ed.)

  • Includes cutting-edge methods and protocols for the study of innate antiviral immunity
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-7237-1
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  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-7236-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.

Table of contents (17 chapters)

  • Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells

    Shiyou Zhu, Yuexin Zhou, Wensheng Wei

    Pages

  • The Application of Humanized Mouse Models for the Study of Human Exclusive Viruses

    Fatemeh Vahedi, Elizabeth C. Giles, Ali A. Ashkar

    Pages

  • Zebrafish as a Model for the Study of Host-Virus Interactions

    Zou, Peng Fei (et al.)

    Pages 57-78

  • Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes

    Long, Tianyun (et al.)

    Pages 79-88

  • Extraction and qPCR-Based Detection of miRNAs from Cultured PBMCs of Bubaline Origin

    Mukhopadhyay, Chandra S. (et al.)

    Pages 89-102

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-7237-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-7236-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Innate Antiviral Immunity
Book Subtitle
Methods and Protocols
Editors
  • Karen Mossman
Series Title
Methods in Molecular Biology
Series Volume
1656
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7237-1
DOI
10.1007/978-1-4939-7237-1
Hardcover ISBN
978-1-4939-7236-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 275
Number of Illustrations and Tables
11 b/w illustrations, 30 illustrations in colour
Topics