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Methods in Molecular Biology

Mammalian Synthetic Promoters

Editors: Gould, David (Ed.)

  • Includes cutting-edge methods protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-7223-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-7221-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.

Table of contents (18 chapters)

  • Initial Considerations Before Designing a Promoter Construct

    Gould, David

    Pages 1-7

  • Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay

    Yousaf, Nasim (et al.)

    Pages 11-21

  • Chromatin Immunoprecipitation and Quantitative Real-Time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites Within a Promoter

    Read, Jordan E.

    Pages 23-32

  • Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo

    Delhove, Juliette M. K. M. (et al.)

    Pages 49-64

  • Monitoring Promoter Activity by Flow Cytometry

    Taher, Taher E. I.

    Pages 65-73

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-7223-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-7221-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mammalian Synthetic Promoters
Editors
  • David Gould
Series Title
Methods in Molecular Biology
Series Volume
1651
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7223-4
DOI
10.1007/978-1-4939-7223-4
Hardcover ISBN
978-1-4939-7221-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 277
Number of Illustrations and Tables
18 b/w illustrations, 25 illustrations in colour
Topics