Hemostasis and Thrombosis

1. Front Matter

   Pages i-xv

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2. Introductory Chapters

   1. Front Matter

      Pages 1-1

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   2. Overview of Hemostasis and Thrombosis and Contribution of Laboratory Testing to Diagnosis and Management of Hemostasis and Thrombosis Disorders

      • Roslyn A. Bonar, Giuseppe Lippi, Emmanuel J. Favaloro

      Pages 3-27

   3. Preanalytical Issues in Hemostasis and Thrombosis Testing

      • Giuseppe Lippi, Emmanuel J. Favaloro

      Pages 29-42

   4. The Interface between Immunotransfusion and Hemostasis and Thrombosis Testing

      • Massimo Franchini

      Pages 43-56

3. Routine Coagulation Assays

   1. Front Matter

      Pages 57-57

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   2. Optimizing the Verification of Mean Normal Prothrombin Time (MNPT) and International Sensitivity Index (ISI) for Accurate Conversion of Prothrombin Time (PT) to International Normalized Ratio (INR)

      • Emmanuel J. Favaloro

      Pages 59-74

   3. Performance of Activated Partial Thromboplastin Time (APTT): Determining Reagent Sensitivity to Factor Deficiencies, Heparin, and Lupus Anticoagulants

      • Geoffrey Kershaw

      Pages 75-83

   4. Performance and Interpretation of Mixing Tests in Coagulation

      • Geoffrey Kershaw

      Pages 85-90

   5. D-Dimer Testing: Laboratory Aspects and Current Issues

      • Jecko Thachil, Giuseppe Lippi, Emmanuel J. Favaloro

      Pages 91-104

   6. Determination of Fibrinogen and Thrombin Time (TT)

      • Anetta Undas

      Pages 105-110

4. Thrombophilia-Related Chapters

   1. Front Matter

      Pages 111-111

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   2. An Overview of Thrombophilia and Associated Laboratory Testing

      • Martina Montagnana, Giuseppe Lippi, Elisa Danese

      Pages 113-135

   3. Laboratory Testing for Activated Protein C Resistance (APCR)

      • Soma Mohammed, Emmanuel J. Favaloro

      Pages 137-143

   4. Assessment of Hereditary Thrombophilia: Performance of Protein C (PC) Testing

      • Richard A. Marlar, Jana N. Gausman

      Pages 145-151

   5. Assessment of Hereditary Thrombophilia: Performance of Protein S (PS) Testing

      • Richard A. Marlar, Jana N. Gausman

      Pages 153-160

   6. Assessment of Hereditary Thrombophilia: Performance of Antithrombin (AT) Testing

      • Jana N. Gausman, Richard A. Marlar

      Pages 161-167

   7. Lupus Anticoagulant Testing: Diluted Russell Viper Venom Time (dRVVT)

      • Vittorio Pengo, Elisa Bison, Alessandra Banzato, Giacomo Zoppellaro, Seena Padayattil Jose, Gentian Denas

      Pages 169-176

   8. Lupus Anticoagulant Testing: Activated Partial Thromboplastin Time (APTT) and Silica Clotting Time (SCT)

      • Armando Tripodi, Veena Chantarangkul

      Pages 177-183

   9. Solid Phase Immunoassay for the Detection of Anticardiolipin Antibodies

      • Rohan Willis, Elizabeth Papalardo, E. Nigel Harris

      Pages 185-199

This collection thoroughly explores the dynamic and ever-developing field of hemostasis and thrombosis diagnostics and research. After an introductory section covering the basics and preanalytical issues, the book continues with in-depth sections that explore how to get the best outcomes from routine coagulation and specialized hemostasis assays, thrombophilia-related techniques, investigations into bleeding disorders, as well as performance of global assays of hemostasis, and finally post-analytical issues in hemostasis and thrombosis testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Hemostasis and Thrombosis: Methods and Protocols serves as an ideal resource for researchers and diagnostic laboratories seeking expert guidance and working to identify the best methodologies to pursue hemostasis and thrombosis testing.

• Coagulation tests
• Thrombophilia
• Bleeding disorders
• Factor XIII
• von Willebrand factor
• Laboratory testing

• Department of Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, Australia

  Emmanuel J. Favaloro

• Section of Clinical Biochemistry, University of Verona, Verona, Italy

  Giuseppe Lippi

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