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  • © 2017

Natural Antibodies

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1643)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid

    • Christoph Schneider, Marlies Illi, Marius Lötscher, Marc Wehrli, Stephan von Gunten
    Pages 23-31
  3. Purification of Natural Antibodies Against Tau Protein by Affinity Chromatography

    • Michala Krestova, Lenka Hromadkova, Jan Ricny
    Pages 33-44
  4. Unbiased RACE-Based Massive Parallel Surveys of Human IgA Antibody Repertoires

    • Hanane El Bannoudi, Céline Anquetil, Marc J. Braunstein, Sergei L. Kosakovsky Pond, Gregg J. Silverman
    Pages 45-73
  5. Analysis of Signaling Events in B-1a Cells

    • Jianxin Huo, Yuhan Huang, Shengli Xu, Kong-Peng Lam
    Pages 75-83
  6. Hydrolysis and Dissolution of Amyloids by Catabodies

    • Ville V. Meretoja, Sudhir Paul, Stephanie A. Planque
    Pages 111-134
  7. Methods for Posttranslational Induction of Polyreactivity of Antibodies

    • Maxime Lecerf, Annaelle Jarossay, Srinivas V. Kaveri, Sébastien Lacroix-Desmazes, Jordan D. Dimitrov
    Pages 135-145
  8. Detection of Natural Antibodies and Serological Diagnosis of Pneumococcal Pneumonia Using a Bead-Based High-Throughput Assay

    • Irene Jiménez-Munguía, Willem J. B. van Wamel, Manuel J. Rodríguez-Ortega, Ignacio Obando
    Pages 169-177
  9. Detection of Naturally Occurring Human Antibodies Against Gangliosides by ELISA

    • Ana María Hernández, Nely Rodríguez-Zhurbenko
    Pages 179-186
  10. Evaluating the Impact of Natural IgM on Adenovirus Type 5 Gene Therapy Vectors

    • Zhili Xu, Jie Tian, Andrew W. Harmon, Andrew P. Byrnes
    Pages 187-196
  11. Erratum to: Hydrolysis and Dissolution of Amyloids by Catabodies

    • Ville V. Meretoja, Sudhir Paul, Stephanie A. Planque
    Pages E1-E1
  12. Back Matter

    Pages 197-199

About this book

This volume looks at the role of natural antibodies in pathogen elimination, cell survival, inflammation, cancer, and autoimmunity. The chapters in this book cover numerous topics, such as isolation of natural antibodies; methods for separating natural antibodies from human plasma, saliva, breast milk, and gastrointestinal fluids; functional properties of natural antibodies such as  anti-tumor cytotoxic activity, and hydrolysis and dissolution of their target antigens; their utility in serological diagnosis of microbial antigens; and the role of natural antibodies in inhibiting viral vectors in the absence of prior exposure to the virus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Natural Antibodies: Methods and Protocols is a valuable resource for researchers and experts who are interested in further studies of natural antibodies.

Editors and Affiliations

  • Centre de Recherche des Cordeliers, INSERM, PARIS, Paris, France

    Srinivas V. Kaveri, Jagadeesh Bayry

Bibliographic Information

  • Book Title: Natural Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Srinivas V. Kaveri, Jagadeesh Bayry

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7180-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7179-4Published: 01 July 2017

  • Softcover ISBN: 978-1-4939-8409-1Published: 12 May 2018

  • eBook ISBN: 978-1-4939-7180-0Published: 30 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 199

  • Number of Illustrations: 11 b/w illustrations, 11 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access