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MicroRNA Technologies

  • Book
  • © 2017

Overview

Editors:
  1. Min Jeong Kye

    1. Institute of Human Genetics, University of Cologne, Cologne, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 128)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord

    • Irit Reichenstein, Eran Hornstein
    Pages 1-10

  3. Protocol for High-Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor Neurons

    • Tal Yardeni, Eran Hornstein
    Pages 11-19

  4. Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-radioactive Fluorescence Method

    • Anna Emde, Natalia Rivkin, Mark A. Behlke, Eran Hornstein
    Pages 21-27

  5. Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Propofol

    • Danielle Twaroski, Yasheng Yan, Jessica M. Olson, Mingyu Liang, Zeljko J. Bosnjak, Xiaowen Bai
    Pages 29-42

  6. MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation

    • Lara Stevanato, Caroline Hicks, Lavaniya Thanabalasundaram, John D. Sinden
    Pages 43-57

  7. Study of miRNA Function in the Developing Axons of Mouse Cortical Neurons: Use of Compartmentalized Microfluidic Chambers and In Utero Electroporation

    • Patricia P. Garcez, Francois Guillemot, Federico Dajas-Bailador
    Pages 59-71

  8. Functional Analysis of Cortical Neuron Migration Using miRNA Silencing

    • Pierre-Paul Prévot, Marie-Laure Volvert, Alexander Deiters, Laurent Nguyen
    Pages 73-88

  9. Continuous Delivery of Oligonucleotides into the Brain

    • Ilya A. Vinnikov, Andrii Domanskyi, Witold Konopka
    Pages 89-117

  10. Isolating and Screening Subcellular miRNAs in Neuron

    • Min Jeong Kye
    Pages 119-127

  11. Experimental Methods for Functional Studies of microRNAs in Animal Models of Psychiatric Disorders

    • Vladimir Jovasevic, Jelena Radulovic
    Pages 129-146

  12. Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs

    • Hak Hee Kim, Monichan Phay, Soonmoon Yoo
    Pages 147-159

  13. In Ovo Electroporation of miRNA Plasmids to Silence Genes in a Temporally and Spatially Controlled Manner

    • Nicole H. Wilson, Esther T. Stoeckli
    Pages 161-181

  14. Three Dimensional Cell Culture of Human Neural Stem Cells Using Polysaccharide-Based Hydrogels and Subsequent Bioanalyses

    • Geun-woo Jin, Weili Ma, Won H. Suh
    Pages 183-204

  15. Measuring miRNA Mediated Translational Regulation with Live Cell Imaging

    • Min Jeong Kye
    Pages 205-211

  16. Erratum to: Experimental Methods for Functional Studies of microRNAs in Animal Models of Psychiatric Disorders

    • Vladimir Jovasevic, Jelena Radulovic
    Pages 213-213

  17. Back Matter

    Pages 215-216
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Keywords

About this book

This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression profiling by PCR array in 2D and 3D neural culture systems; analysis of cortical neuron migration using miRNA silencing; isolating and screening subcellular miRNAs in neurons; and measuring miRNA mediated translational regulation with live cell imaging. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Cutting-edge and practical, MicroRNA Technologies is a valuable resource for students and researchers who are interested about microRNAs in nervous systems.

Editors and Affiliations

  • Institute of Human Genetics, University of Cologne, Cologne, Germany

    Min Jeong Kye

Bibliographic Information

  • Book Title: MicroRNA Technologies

  • Editors: Min Jeong Kye

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-7175-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7173-2Published: 26 July 2017

  • Softcover ISBN: 978-1-4939-8407-7Published: 02 August 2018

  • eBook ISBN: 978-1-4939-7175-6Published: 25 July 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 216

  • Number of Illustrations: 10 b/w illustrations, 10 illustrations in colour

  • Topics: Neurosciences

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