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Plant Argonaute Proteins

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Alberto Carbonell

    1. Instituto de Biología Molecular y Celular de Plantas, (Consejo Superior de Investigaciones Científicas–Universidad Politécnica de Valencia), Valencia, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols.
  • Provides step-by-step essential for reproducible results.
  • Contains key notes and implementation advice from the expert
  • Includes supplementary material: sn.pub/extras
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1640)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Plant ARGONAUTEs: Features, Functions, and Unknowns

    • Alberto Carbonell
    Pages 1-21

  3. Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants

    • Guodong Ren, Xiaoyan Wang, Bin Yu
    Pages 23-37

  4. In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts

    • Taichiro Iki, Masayuki Ishikawa, Manabu Yoshikawa
    Pages 39-53

  5. In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants

    • Yukihide Tomari, Hiro-oki Iwakawa
    Pages 55-71

  6. Establishment of an In Vivo ARGONAUTE Reporter System in Plants

    • Károly Fátyol, József Burgyán
    Pages 73-91

  7. Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants

    • Alberto Carbonell
    Pages 93-112

  8. Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing

    • Ivett Baksa, György Szittya
    Pages 113-128

  9. Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants

    • Cheng-Guo Duan, Jian-Kang Zhu
    Pages 129-135

  10. Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana

    • Cécile Lecampion, Elodie Lanet, Christophe Robaglia
    Pages 137-143

  11. Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair

    • Marina Martinez-Garcia, Mónica Pradillo
    Pages 145-158

  12. Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas

    • Tomohito Yamasaki
    Pages 159-172

  13. ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation

    • Meizhen Wang, Yuxing Deng, Fenjuan Shao, Miaomiao Liu, Yongqi Pang, Caili Li et al.
    Pages 173-189

  14. Cloning and Characterization of Argonaute Genes in Tomato

    • Zhiqiang Xian, Fang Yan, Zhengguo Li
    Pages 191-197

  15. Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes

    • Ariel H. Tomassi, Delfina Gagliardi, Damian A. Cambiagno, Pablo A. Manavella
    Pages 199-210

  16. Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes

    • Jun Hu, Yingguo Zhu
    Pages 211-217

  17. Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants

    • Kai Tang, Cheng-Guo Duan, Huiming Zhang, Jian-Kang Zhu
    Pages 219-225

  18. Structural and Functional Characterization of Plant ARGONAUTE MID Domains

    • Filipp Frank, Bhushan Nagar
    Pages 227-239

  19. Identification and Analysis of WG/GW ARGONAUTE-Binding Domains

    • Andrzej Zielezinski, Wojciech M. Karlowski
    Pages 241-256

  20. Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms

    • Joshua T. Trujillo, Rebecca A. Mosher
    Pages 257-266
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Keywords

About this book

This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.

Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.

Editors and Affiliations

  • Instituto de Biología Molecular y Celular de Plantas, (Consejo Superior de Investigaciones Científicas–Universidad Politécnica de Valencia), Valencia, Spain

    Alberto Carbonell

Bibliographic Information

  • Book Title: Plant Argonaute Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Alberto Carbonell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7165-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7164-0Published: 13 June 2017

  • Softcover ISBN: 978-1-4939-8404-6Published: 12 August 2018

  • eBook ISBN: 978-1-4939-7165-7Published: 12 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 297

  • Number of Illustrations: 20 b/w illustrations, 27 illustrations in colour

  • Topics: Plant Sciences

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