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Acute Myeloid Leukemia

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Paolo Fortina

    1. Thomas Jefferson University, Sidney Kimmel Cancer Center, Philadelphia, USA

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  2. Eric Londin

    1. Department of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College Thomas Jefferson University, Philadelphia, USA

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  3. Jason Y. Park

    1. Department of Pathology, UT Southwestern Medical Center and Children’s Medical Center, Dallas, USA

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  4. Larry J. Kricka

    1. Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1633)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Molecular Malfeasance Mediating Myeloid Malignancies: The Genetics of Acute Myeloid Leukemia

    • Rebecca L. King, Adam Bagg
    Pages 1-17

  3. Cytochemical Staining

    • Michele E. Paessler, Marybeth Helfrich, Gerald B. W. Wertheim
    Pages 19-32

  4. Immunohistochemistry in Acute Myeloid Leukemia

    • Michael W. Cruise
    Pages 33-49

  5. Immunophenotyping by Multiparameter Flow Cytometry

    • Weina Chen, Hung S. Luu
    Pages 51-73

  6. Single-Cell Mass Cytometry of Acute Myeloid Leukemia and Leukemia Stem/Progenitor Cells

    • Zhihong Zeng, Marina Konopleva, Michael Andreeff
    Pages 75-86

  7. Design and Application of Multiplex PCR Seq for the Detection of Somatic Mutations Associated with Myeloid Malignancies

    • Naomi Park, George Vassiliou
    Pages 87-99

  8. AMLprofiler: A Diagnostic and Prognostic Microarray for Acute Myeloid Leukemia

    • Marco Alessandrini, Sharon S. Kappala, Michael S. Pepper
    Pages 101-123

  9. Microsphere-Based Assessment of DNA Methylation for AML Prognosis

    • Gerald B. W. Wertheim, Marlise R. Luskin, Martin Carroll, Stephen R. Master
    Pages 125-136

  10. Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS

    • Mira Jeong, Anna G. Guzman, Margaret A. Goodell
    Pages 137-149

  11. Detection and Quantification of Acute Myeloid Leukemia-Associated Fusion Transcripts

    • Jonathan Schumacher, Philippe Szankasi, Todd W. Kelley
    Pages 151-161

  12. Targeted Next-Generation Sequencing of Acute Leukemia

    • Eric Konnick, Christina M. Lockwood, David Wu
    Pages 163-184

  13. Bioinformatics Data Analysis of Next-Generation Sequencing Data from Heterogeneous Tumor Samples

    • Sean R. Landman, Tae Hyun Hwang
    Pages 185-192

  14. A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation

    • Alvin C. H. Ma, Xiangguo Shi, Bai-Liang He, Yuhan Guo, Anskar Y. H. Leung
    Pages 193-218

  15. Measurement of Oncometabolites d-2-Hydroxyglutaric Acid and l-2-Hydroxyglutaric Acid

    • Patricia M. Jones, Richard Boriack, Eduard A. Struys, Dinesh Rakheja
    Pages 219-234

  16. Nanofluidic Allele-Specific Digital PCR Method for Quantifying IDH1 and IDH2 Mutation Burden in Acute Myeloid Leukemia

    • Daniel H. Wiseman, Tim C. P. Somervaille
    Pages 235-255

  17. Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer

    • Chang-Sook Hong, Sonja Funk, Theresa L. Whiteside
    Pages 257-265

  18. Generating and Expanding Autologous Chimeric Antigen Receptor T Cells from Patients with Acute Myeloid Leukemia

    • Saad S. Kenderian, Carl H. June, Saar Gill
    Pages 267-276

  19. Back Matter

    Pages 277-278
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Keywords

About this book

This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.

Editors and Affiliations

  • Thomas Jefferson University, Sidney Kimmel Cancer Center, Philadelphia, USA

    Paolo Fortina

  • Department of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College Thomas Jefferson University, Philadelphia, USA

    Eric Londin

  • Department of Pathology, UT Southwestern Medical Center and Children’s Medical Center, Dallas, USA

    Jason Y. Park

  • Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, USA

    Larry J. Kricka

Bibliographic Information

  • Book Title: Acute Myeloid Leukemia

  • Book Subtitle: Methods and Protocols

  • Editors: Paolo Fortina, Eric Londin, Jason Y. Park, Larry J. Kricka

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7142-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7140-4Published: 23 July 2017

  • Softcover ISBN: 978-1-4939-8396-4Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7142-8Published: 22 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 278

  • Number of Illustrations: 3 b/w illustrations, 43 illustrations in colour

  • Topics: Cancer Research

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