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Current Proteomic Approaches Applied to Brain Function

  • Book
  • © 2017

Overview

Editors:
  1. Enrique Santamaría

    1. Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

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  2. Joaquín Fernández-Irigoyen

    1. Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 127)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Brain Proteomics: Decoding Neuroproteomes Using Mass Spectrometry

      • Joaquín Fernández-Irigoyen, Enrique Santamaría
      Pages 3-7

  3. Labeling Methods in Neuroproteomics

    1. Front Matter

      Pages 9-9
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    2. Applications of Amine-Reactive Tandem Mass Tags (TMT) in Human Neuroproteomics

      • Linnéa Lagerstedt, Leire Azurmendi, Jean-Charles Sanchez
      Pages 11-28

    3. Application of Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to Monitor Olfactory Proteomes During Alzheimer’s Disease Progression

      • Andrea González Morales, Mercedes Lachén-Montes, María Ibáñez-Vea, Enrique Santamaría, Joaquín Fernández-Irigoyen
      Pages 29-42

    4. Protein Microarrays in Neurodegenerative Diseases

      • Pablo San Segundo-Acosta, María Garranzo-Asensio, Ana Montero-Calle, Carmen Oeo-Santos, Mayte Villalba, Ana Guzman-Aranguez et al.
      Pages 43-62

  4. Label-Free Methods in Neuroproteomics

    1. Front Matter

      Pages 63-63
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    2. Comprehensive Shotgun Proteomic Analyses of Oligodendrocytes Using Ion Mobility and Data-Independent Acquisition

      • Juliana S. Cassoli, Daniel Martins-de-Souza
      Pages 65-74

    3. Nontargeted Brain Lipidomic Profiling Performed by UPLC-ESI-qToF-MS/MS

      • Alba Naudí, Rosanna Cabré, Mariona Jové, Reinald Pamplona
      Pages 75-90

    4. Methods for Human Olfactory Bulb Tissue Studies Using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS)

      • Ibon Iloro, Joaquín Fernández-Irigoyen, Iraide Escobes, Mikel Azkargorta, Enrique Santamaría, Felix Elortza
      Pages 91-106

    5. Neuroproteomics Using Short GeLC-SWATH: From the Evaluation of Proteome Changes to the Clarification of Protein Function

      • Sandra I. Anjo, Cátia Santa, Susana C. Saraiva, Karolina Freitas, Faraj Barah, Bruno Carreira et al.
      Pages 107-138

  5. Mass Spectrometry-Based Neuroproteomics to Analyze Post-Translational Modificiations

    1. Front Matter

      Pages 139-139
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    2. Analysis of Brain Phosphoproteome Using Titanium Dioxide Enrichment and High-Resolution LC-MS/MS

      • Jeffrey M. Sifford, Haiyan Tan, Hong Wang, Junmin Peng
      Pages 141-159

    3. N-Glycomics and N-Glycoproteomics of Human Cerebrospinal Fluid

      • Sophie Cholet, Arnaud Goyallon, Christophe Junot, François Fenaille
      Pages 161-177

    4. In Vivo Strategies to Isolate and Characterize the Neuronal Ubiquitinated Proteome

      • Juanma Ramirez, Nagore Elu, Aitor Martinez, Benoit Lectez, Ugo Mayor
      Pages 179-189

    5. Characterization of the Phosphoproteome and Sialoproteome in Brain Tissues by Mass Spectrometry

      • María Ibáñez-Vea, Stefan J. Kempf, Martin R. Larsen
      Pages 191-206

    6. Proteomic Analysis of SUMOylation in the Post-ischemic Brain

      • J. Will Thompson, Meng Jiang, Wei Yang
      Pages 207-224

    7. S-Nitrosylation in Alzheimer’s Disease Using Oxidized Cysteine-Selective cPILOT

      • Ryan R. Dyer, Liqing Gu, Renã A. S. Robinson
      Pages 225-241

  6. Subcellular Neuroproteomics

    1. Front Matter

      Pages 243-243
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Keywords

About this book

This volume focuses on protein analysis covering a wide spectrum of the utility of mass spectrometry within neurobiological disciplines. The chapters in this book discuss label (iTRAQ, TMT, protein arrays) and label-free workflows (SWATH, MALDI Imaging, and label-free quantitation). Other chapters look at experimental strategies targeted to the identification and quantitation of specific lipids and post-translational modifications, as well as proteomic workflows that focus on characterization of subcellular proteomes. The last few chapters in the book describe various bioinformatics pipelines used to analyze the molecular data derived from high-throughput transcriptomic and proteomic experiments on brain tissue. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Thorough and comprehensive, Current Proteomic Approaches Applied to Brain Function is a valuable resource for graduate students and postdoctoral fellows interested in neuroproteomics, as well as researchers looking for further insight into the growing field of mass spectrometry in neuroscience.


Editors and Affiliations

  • Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

    Enrique Santamaría, Joaquín Fernández-Irigoyen

Bibliographic Information

  • Book Title: Current Proteomic Approaches Applied to Brain Function

  • Editors: Enrique Santamaría, Joaquín Fernández-Irigoyen

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-7119-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7118-3Published: 27 June 2017

  • Softcover ISBN: 978-1-4939-8390-2Published: 07 August 2018

  • eBook ISBN: 978-1-4939-7119-0Published: 25 June 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XVI, 360

  • Number of Illustrations: 30 b/w illustrations, 61 illustrations in colour

  • Topics: Neurosciences

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