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Ebolaviruses

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Thomas Hoenen

    1. Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Allison Groseth

    1. Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of ebolaviruses
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
  • Includes supplementary material: sn.pub/extras
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1628)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvi
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  3. Studying Ebolaviruses Under Biosafety Level 2 Conditions

    1. Front Matter

      Pages 51-51
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    2. Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms

      • Rachel B. Brouillette, Wendy Maury
      Pages 53-63

    3. Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins

      • Patrick L. Sinn, Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt, Wendy Maury
      Pages 65-78

    4. Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems

      • Tessa Cressey, Kristina Brauburger, Elke Mühlberger
      Pages 79-92

    5. Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation

      • Logan Banadyga, Hideki Ebihara
      Pages 93-107

    6. Modeling Ebolavirus Budding with Virus Like Particles

      • Olivier Reynard, Mathieu Mateo
      Pages 109-117

    7. Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Viruslike Particle Assays

      • Nadine Biedenkopf, Thomas Hoenen
      Pages 119-131

    8. Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins

      • Priya Luthra, Christopher F. Basler
      Pages 133-142

    9. Nonradioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA

      • Kristina Brauburger, Tessa Cressey, Elke Mühlberger
      Pages 143-159

  4. Studying Infectious Ebolaviruses In Vitro

    1. Front Matter

      Pages 161-161
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    2. A Semi-automated High-Throughput Microtitration Assay for Filoviruses

      • Claire Marie Filone, David Miller, Victoria Wahl-Jensen
      Pages 163-175

    3. Generation of Recombinant Ebola Viruses Using Reverse Genetics

      • Allison Groseth
      Pages 177-188

    4. Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals

      • Thomas Hoenen
      Pages 189-194

    5. Live-Cell Imaging of Filoviruses

      • Gordian Schudt, Olga Dolnik, Stephan Becker
      Pages 195-202

    6. Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds

      • Darryl Falzarano
      Pages 203-210
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Keywords

About this book

This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena.

Reviews

“This book comprises three review chapters on filoviruses and 27 protocol chapters. … Scientists working on filoviruses and those wishing to enter the field will find a compendium of carefully written methods and protocols relevant to their work. … What makes the book stand out from many other volumes in this series is the inclusion of the three review chapters, which by themselves earn the book a spot on the bookshelf of any scientist working in the field.” (Christoph Wirblich, Doody's Book Reviews, January, 2018)

Editors and Affiliations

  • Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

    Thomas Hoenen, Allison Groseth

Bibliographic Information

  • Book Title: Ebolaviruses

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Hoenen, Allison Groseth

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7116-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7115-2Published: 01 June 2017

  • Softcover ISBN: 978-1-4939-8389-6Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7116-9Published: 01 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 397

  • Number of Illustrations: 13 b/w illustrations, 55 illustrations in colour

  • Topics: Infectious Diseases

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