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Methods in Molecular Biology

Cryopreservation of Mammalian Gametes and Embryos

Methods and Protocols

Editors: Nagy, Zsolt Peter, Varghese, Alex C., Agarwal, Ashok (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-6828-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-6826-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation. 

About the authors

Dr. Ashok Agarwal is the Head of the Clinical Andrology Center, which over the years under his leadership, has become a center of excellence in the United States for the diagnosis of male infertility and fertility preservation of men with oncological conditions. He is board certified as a Clinical Laboratory Director in Andrology by the American Board of Bioanalysis and an Inspector for the College of American Pathologists “Reproductive Laboratory Program” for accreditation of Andrology & IVF Laboratories. Dr. Agarwal is the recipient of several awards, such as the 2011 Innovator Award from Cleveland Clinic Innovations for the development of “Remote Sperm Banking Kits.” His current research interests are identifying biological markers of oxidative stress, DNA damage, and apoptosis using proteomic research tools and bioinformatics analysis, as well as preserving fertility in patients with cancer.
Zsolt Peter Nagy, M.D., Ph.D., is the Scientific Director at Reproductive Biology Associates (RBA), in Atlanta, GA, USA. He has acquired distinctive knowledge and experience on embryo science. He is well known for his contributions to oocyte vitrification and the development of the largest donor oocyte cryo-bank in North America. Dr. Nagy is a member/officer of several national and international professional societies, including ASRM, ESHRE, Alpha, ISFP and AFP, as well as a reviewer and editor. He is the author and/or co-author of over 200 publications, book chapters, and books.
Dr. Alex C. Varghese is the Scientific and Laboratory Director at Astra Fertility group in Mississauga, Canada. His proficiency and vast experience in clinical embryology and academics earned him a reputation in the ART community. He is well regarded for his passion to disseminate knowledge through online platforms and also as a leader of embryology society. He has helped many units establish training programs and affordable batch IVF units in many South Asian Countries. His area of interest is in developing automated systems for assisted reproduction. Dr. Varghese believes in “responsible reproduction” through ethical practices, low-cost IVF for the masses, quality management, and safe practices that safe guard the health of IVF children.

Table of contents (27 chapters)

  • Chapter 1 Historical Background on Gamete and Embryo Cryopreservation

    Ali, Jaffar (et al.)

    Pages 3-20

  • Chapter 2 Utility of Animal Models for Human Ovarian Tissue Cryopreservation

    Kim, Seul Ki (et al.)

    Pages 23-31

  • Chapter 3 Current Challenges in Immature Oocyte Cryopreservation

    Chian, Ri-Cheng (et al.)

    Pages 33-44

  • Chapter 4 Role of Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification

    Kim, Seul Ki (et al.)

    Pages 45-63

  • Chapter 5 Slow Freezing of Human Sperm

    Agarwal, Ashok (et al.)

    Pages 67-78

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-6828-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-6826-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cryopreservation of Mammalian Gametes and Embryos
Book Subtitle
Methods and Protocols
Editors
  • Zsolt Peter Nagy
  • Alex C. Varghese
  • Ashok Agarwal
Series Title
Methods in Molecular Biology
Series Volume
1568
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6828-2
DOI
10.1007/978-1-4939-6828-2
Hardcover ISBN
978-1-4939-6826-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 372
Number of Illustrations and Tables
26 b/w illustrations, 119 illustrations in colour
Topics