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Methods in Molecular Biology

Morpholino Oligomers

Methods and Protocols

Editors: Moulton, Hong M, Moulton, Jon D. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-6817-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-6815-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier.


Table of contents (22 chapters)

  • Invention and Early History of Morpholinos: From Pipe Dream to Practical Products

    Summerton, James E.

    Pages 1-15

  • Making a Morpholino Experiment Work: Controls, Favoring Specificity, Improving Efficacy, Storage, and Dose

    Moulton, Jon D.

    Pages 17-29

  • Aggregation and Disaggregation of Morpholino Oligomers in Solution

    Chow, Garrick (et al.)

    Pages 31-38

  • End-Modifications on Morpholino Oligos

    Li, Yong-Fu

    Pages 39-50

  • Inducible Inhibition of Gene Function with Photomorpholinos

    Sumanas, Saulius

    Pages 51-57

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-6817-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-6815-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Morpholino Oligomers
Book Subtitle
Methods and Protocols
Editors
  • Hong M Moulton
  • Jon D. Moulton
Series Title
Methods in Molecular Biology
Series Volume
1565
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6817-6
DOI
10.1007/978-1-4939-6817-6
Hardcover ISBN
978-1-4939-6815-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 284
Number of Illustrations and Tables
22 b/w illustrations, 38 illustrations in colour
Topics