Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1563)
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Table of contents (17 protocols)
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Front Matter
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Advanced Fluorescence Microscopy: Sample Preparation, Fluorophores and Model Systems
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Front Matter
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Super and High-Resolution Optical Imaging
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Front Matter
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Quantitative and Computational Image Analysis
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Front Matter
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About this book
This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imaging. Light Microscopy: Methods and Protocols puts special focus on system instrumentation parameters and sophisticated labeling and detection methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Light Microscopy: Methods and Protocols offers the novice user with straightforward strategies to address biological questions, while providing the experienced researcher with the latest applications that can be useful in routine practices. This book also serves as a useful teaching manual in practical courses on light microscopy.
Keywords
- confocal laser scanning and spinning disk confocal microscopy
- Fluorescence Resonance Energy Transfer (FRET)
- Fluorescence Recovery After Photobleaching (FRAP)
- light-sheet and two-photon excitation microscopy
- Photoactivated Localization Microscopy (PALM)
- Stimulated Emission Depletion (STED)
- Total Internal Reflection Fluorescence (TIRF)
- biomolecular spatiotemporal dynamics
Reviews
Editors and Affiliations
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Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, USA
Yolanda Markaki
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Biozentrum der LMU Munchen, Planegg-Martinsried, Germany
Hartmann Harz
Bibliographic Information
Book Title: Light Microscopy
Book Subtitle: Methods and Protocols
Editors: Yolanda Markaki, Hartmann Harz
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6810-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6808-4Published: 05 April 2017
Softcover ISBN: 978-1-4939-8305-6Published: 20 July 2018
eBook ISBN: 978-1-4939-6810-7Published: 21 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 285
Number of Illustrations: 11 b/w illustrations, 62 illustrations in colour
Topics: Imaging / Radiology