Methods in Molecular Biology

Light Microscopy

Methods and Protocols

Editors: Markaki, Yolanda, Harz, Hartmann (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-6810-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-6808-4
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imaging. Light Microscopy: Methods and Protocols puts special focus on system instrumentation parameters and sophisticated labeling and detection methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Light Microscopy: Methods and Protocols offers the novice user with straightforward strategies to address biological questions, while providing the experienced researcher with the latest applications that can be useful in routine practices. This book also serves as a useful teaching manual in practical courses on light microscopy.

Reviews

“This well-illustrated book provides up-to-date light microscopy approaches and tool sets offered for live or fixed cell observations with a focus on system instrumentation parameters and sophisticated labeling and detection methods. … authors hope that this book will prove to be a robust teaching guide for light microscopy practical courses. I echo the authors’ opinion. … an excellent book which may serve as a user manual on current light microscopy approaches and toolsets for live or fixed cell observations.” (Omer Iqbal, Doody's Book Reviews, July, 2017)


Table of contents (17 chapters)

  • Introduction to Modern Methods in Light Microscopy

    Ryan, Joel (et al.)

    Pages 1-15

  • Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM)

    Pampaloni, Francesco (et al.)

    Pages 19-31

  • Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy

    Jährling, Nina (et al.)

    Pages 33-49

  • Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis

    Grinsven, Erinke (et al.)

    Pages 51-71

  • Imaging of Brain Slices with a Genetically Encoded Voltage Indicator

    Quicke, Peter (et al.)

    Pages 73-84

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-6810-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-6808-4
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Light Microscopy
Book Subtitle
Methods and Protocols
Editors
  • Yolanda Markaki
  • Hartmann Harz
Series Title
Methods in Molecular Biology
Series Volume
1563
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6810-7
DOI
10.1007/978-1-4939-6810-7
Hardcover ISBN
978-1-4939-6808-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 285
Number of Illustrations and Tables
11 b/w illustrations, 62 illustrations in colour
Topics