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  • © 2017

Histochemistry of Single Molecules

Methods and Protocols

  • Includes cutting-edge techniques for the study of single molecule histochemistry
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1560)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
  2. Vital Histochemistry

    1. Front Matter

      Pages 1-1
    2. Histochemical Demonstration of Tripeptidyl Aminopeptidase I

      • Mashenka B. Dimitrova, Dimitrinka Y. Atanasova, Nikolai E. Lazarov
      Pages 55-68
  3. Lectin Histochemistry

    1. Front Matter

      Pages 91-91
    2. Lectin Histochemistry for Metastasizing and Non-metastasizing Cancer Cells

      • Gerrit Wolters-Eisfeld, Udo Schumacher
      Pages 121-132
    3. The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

      • Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Martínez-Hernández, Concepción Ferrer, Luis Miguel Pastor
      Pages 133-144
  4. Protein Histochemistry

    1. Front Matter

      Pages 145-145
  5. Lipid Histochemistry

    1. Front Matter

      Pages 195-195
    2. Tissue Fixation and Processing for the Histological Identification of Lipids

      • Víctor Carriel, Fernando Campos, José Aneiros-Fernández, John A. Kiernan
      Pages 197-206
    3. Staining Methods for Normal and Regenerative Myelin in the Nervous System

      • Víctor Carriel, Antonio Campos, Miguel Alaminos, Stefania Raimondo, Stefano Geuna
      Pages 207-218

About this book

This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets.  It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA  fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.

Editors and Affiliations

  • Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Carlo Pellicciari, Marco Biggiogera

Bibliographic Information

  • Book Title: Histochemistry of Single Molecules

  • Book Subtitle: Methods and Protocols

  • Editors: Carlo Pellicciari, Marco Biggiogera

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6788-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6787-2Published: 03 February 2017

  • Softcover ISBN: 978-1-4939-8301-8Published: 17 July 2018

  • eBook ISBN: 978-1-4939-6788-9Published: 21 January 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 341

  • Number of Illustrations: 11 b/w illustrations, 59 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access