Inflammation

1. Front Matter

   Pages i-xvi

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2. Reproducibility Issues: Avoiding Pitfalls in Animal Inflammation Models

   • Jon D. Laman, Susanne M. Kooistra, Björn E. Clausen

   Pages 1-17

3. Skin Inflammation

   1. Front Matter

      Pages 19-19

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   2. Hapten-Specific T Cell-Mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate

      • Nicolas Bouladoux, Clotilde Hennequin, Camille Malosse, Bernard Malissen, Yasmine Belkaid, Sandrine Henri

      Pages 21-36

   3. Monitoring Skin Dendritic Cells in Steady State and Inflammation by Immunofluorescence Microscopy and Flow Cytometry

      • Julia L. Ober-Blöbaum, Daniela Ortner, Bernhard Haid, Anna Brand, Christoph Tripp, Björn E. Clausen et al.

      Pages 37-52

   4. Visualization of the T Cell Response in Contact Hypersensitivity

      • Gyohei Egawa, Kenji Kabashima

      Pages 53-62

   5. Ultraviolet Radiation-Induced Immunosuppression: Induction of Regulatory T Cells

      • A. Bruhs, T. Schwarz

      Pages 63-73

   6. Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Model

      • Armanda J. Onderdijk, Ineke M. Hekking-Weijma, Edwin F. Florencia, Errol P. Prens

      Pages 75-81

   7. Xenotransplantation Model of Psoriasis

      • Jeremy Di Domizio, Curdin Conrad, Michel Gilliet

      Pages 83-90

   8. A Mouse Model for Atopic Dermatitis Using Topical Application of Vitamin D3 or of Its Analog MC903

      • Verena Moosbrugger-Martinz, Matthias Schmuth, Sandrine Dubrac

      Pages 91-106

   9. Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

      • Ena Sokol, Miranda Nijenhuis, Klaas A. Sjollema, Marcel F. Jonkman, Hendri H. Pas, Ben N. G. Giepmans

      Pages 107-118

4. Inflammation of the Lung

   1. Front Matter

      Pages 119-119

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   2. Murine Models of Allergic Asthma

      • Eline Haspeslagh, Nincy Debeuf, Hamida Hammad, Bart N. Lambrecht

      Pages 121-136

   3. Subcutaneous and Sublingual Immunotherapy in a Mouse Model of Allergic Asthma

      • Laura Hesse, Martijn C. Nawijn

      Pages 137-168

   4. Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse

      • Bobby W. S. Li, Dior M. J. M. Beerens, Maarten D. Brem, Rudi W. Hendriks

      Pages 169-183

   5. Induction and Analysis of Bronchus-Associated Lymphoid Tissue

      • Henrike Fleige, Reinhold Förster

      Pages 185-198

   6. Messenger RNA Sequencing of Rare Cell Populations in the Lung and Lung-Draining Lymph Nodes

      • Alexander Ulges, Edgar Schmitt, Tobias Bopp, Matthias Klein

      Pages 199-219

5. Inflammatory Diseases of the Intestinal and Oral Mucosa

   1. Front Matter

      Pages 221-221

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   2. Isolation and Identification of Intestinal Myeloid Cells

      • Charlotte L. Scott, Calum C. Bain, Allan McI Mowat

      Pages 223-239

   3. Monitoring and Modulation of Inducible Foxp3+ Regulatory T-Cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon

      • S. Veenbergen, L. A. van Berkel, M. F. du Pré, A. E. Kozijn, Janneke N. Samsom

      Pages 241-254

This volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated inflammatory disease models affecting barrier organs to the environment; the skin, the lung, and the intestinal and oral mucosa. The fourth section illustrates inflammatory disease models of the brain, joints, and vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Inflammation: Methods and Protocols aims to inspire the experienced investigator and the young experimenter alike to disentangle the fascinating process of inflammation.

• human IMID
• characterization of leukocytes
• CyTOF
• RNA sequencing
• inflammatory disease models

• Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

  Björn E. Clausen

• Section Medical Physiology Department of Neuroscience, University of Groningen University Medical Center Groningen, Groningen, The Netherlands

  Jon D. Laman

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