Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1498)
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Table of contents (34 protocols)
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Front Matter
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Gene and Genome-Editing Methods Part I
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Front Matter
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Gene and Genome-Editing Methods Part II
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Front Matter
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Bioinformatics Approaches for Identifying and Analyzing Mutagenesis Targets
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Front Matter
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About this book
In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.
Editors and Affiliations
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Coskata, Inc., Warrenville, USA
Andrew Reeves
Bibliographic Information
Book Title: In Vitro Mutagenesis
Book Subtitle: Methods and Protocols
Editors: Andrew Reeves
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6472-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6470-3Published: 06 October 2016
Softcover ISBN: 978-1-4939-8211-0Published: 27 June 2018
eBook ISBN: 978-1-4939-6472-7Published: 05 October 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 511
Number of Illustrations: 44 b/w illustrations, 52 illustrations in colour
Topics: Human Genetics