Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1486)
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Table of contents (21 protocols)
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Front Matter
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Theory and Design of Optical Tweezers
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Front Matter
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Optical Tweezers-Based Manipulation and Analysis of DNA-DNA Interactions, Protein Unfolding/Refolding, and Motion/Force-Generation by Mechanoenzymes In Vitro
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Front Matter
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About this book
The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines.
Editors and Affiliations
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Department of Anatomy and Structural Biology & Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, USA
Arne Gennerich
Bibliographic Information
Book Title: Optical Tweezers
Book Subtitle: Methods and Protocols
Editors: Arne Gennerich
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6421-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6419-2Published: 15 November 2016
Softcover ISBN: 978-1-4939-8194-6Published: 23 June 2018
eBook ISBN: 978-1-4939-6421-5Published: 14 November 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 555
Number of Illustrations: 21 b/w illustrations, 133 illustrations in colour
Topics: Biotechnology