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  • © 2016

Capillary Electrophoresis

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1483)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
  2. Principles and Instrumental

    1. Front Matter

      Pages 1-1
    2. The CE-Way of Thinking: “All Is Relative!”

      • Philippe Schmitt-Kopplin, Agnes Fekete
      Pages 3-19
    3. Derivatization in Capillary Electrophoresis

      • M. Luisa Marina, María Castro-Puyana
      Pages 37-52
    4. Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE–MS Peptide Analysis: Strategies and Implementation

      • Martin Pattky, Katalin Barkovits, Katrin Marcus, Oliver H. Weiergräber, Carolin Huhn
      Pages 53-75
    5. Micellar Electrokinetic Chromatography of Aminoglycosides

      • Ulrike Holzgrabe, Stefanie Schmitt, Frank Wienen
      Pages 77-90
    6. Microemulsion Electrokinetic Chromatography

      • Wolfgang Buchberger
      Pages 91-109
    7. Nonaqueous Capillary Electrophoresis Mass Spectrometry

      • Christian W. Klampfl, Markus Himmelsbach
      Pages 111-130
    8. Ionic Liquids in Capillary Electrophoresis

      • Ulrike Holzgrabe, Joachim Wahl
      Pages 131-153
    9. CZE–CZE ESI–MS Coupling with a Fully Isolated Mechanical Valve

      • Felix J. Kohl, Christian Neusüß
      Pages 155-166
  3. Applications from Small to Macromolecules

    1. Front Matter

      Pages 195-195
    2. Analysis of Small Ions with Capillary Electrophoresis

      • Jatinder Singh Aulakh, Ramandeep Kaur, Ashok Kumar Malik
      Pages 197-216
    3. Metal Ions Analysis with Capillary Zone Electrophoresis

      • Ashok Kumar Malik, Jatinder Singh Aulakh, Varinder Kaur
      Pages 217-247
    4. Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis

      • Daniela Fico, Antonio Pennetta, Giuseppe E. De Benedetto
      Pages 249-276
    5. Enantiomer Separations by Capillary Electrophoresis

      • Gerhard K. E. Scriba, Henrik Harnisch, Qingfu Zhu
      Pages 277-299
    6. Capillary Electrophoresis of Mono- and Oligosaccharides

      • Mila Toppazzini, Anna Coslovi, Marco Rossi, Anna Flamigni, Edi Baiutti, Cristiana Campa
      Pages 301-338
    7. Use of Capillary Electrophoresis for Polysaccharide Studies and Applications

      • Amelia Gamini, Anna Coslovi, Mila Toppazzini, Isabella Rustighi, Cristiana Campa, Amedeo Vetere et al.
      Pages 339-363

About this book

This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.

 

Editors and Affiliations

  • Helmholtz Zentrum Munchen, Neuherberg, Germany

    Philippe Schmitt-Kopplin

Bibliographic Information

  • Book Title: Capillary Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Philippe Schmitt-Kopplin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6403-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-6401-7Published: 20 September 2016

  • Softcover ISBN: 978-1-4939-8188-5Published: 20 June 2018

  • eBook ISBN: 978-1-4939-6403-1Published: 19 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 527

  • Number of Illustrations: 128 b/w illustrations, 28 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access