High-Throughput Screening Assays in Toxicology

1. Front Matter

   Pages i-xii

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2. In Vitro Toxicological High-Thoughput Screening Methods

   1. Front Matter

      Pages 1-1

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   2. Monitoring Ligand-Activated Protein–Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay

      • Carlos Coriano, Emily Powell, Wei Xu

      Pages 3-15

   3. Mitochondrial Membrane Potential Assay

      • Srilatha Sakamuru, Matias S. Attene-Ramos, Menghang Xia

      Pages 17-22

   4. Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway

      • Thai Khuc, Chia-Wen (Amy) Hsu, Srilatha Sakamuru, Menghang Xia

      Pages 23-31

   5. Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators

      • Caitlin Lynch, Jinghua Zhao, Hongbing Wang, Menghang Xia

      Pages 33-42

   6. Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity

      • Chia-Wen (Amy) Hsu, Jinghua Zhao, Menghang Xia

      Pages 43-53

   7. Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway

      • Jinghua Zhao, Sunita J. Shukla, Menghang Xia

      Pages 55-62

   8. Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells

      • Yitong Liu

      Pages 63-70

   9. Determination of Histone H2AX Phosphorylation in DT40 Cells

      • Kana Nishihara, Sampada A. Shahane, Menghang Xia

      Pages 71-76

   10. High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells

       • Sampada A. Shahane, Kana Nishihara, Menghang Xia

       Pages 77-85

3. In Vivo Toxicological High-Thoughput Screening Methods

   1. Front Matter

      Pages 87-87

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   2. Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish

      • Lisa Truong, Michael T. Simonich, Robert L. Tanguay

      Pages 89-98

   3. Fast Functional Germline and Epigenetic Assays in the Nematode Caenorhabditis elegans

      • Zachary Lundby, Jessica Camacho, Patrick Allard

      Pages 99-107

4. In Silico High-Thoughput Screening Toxcity Data Analysis

   1. Front Matter

      Pages 109-109

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   2. A Quantitative High-Throughput Screening Data Analysis Pipeline for Activity Profiling

      • Ruili Huang

      Pages 111-122

   3. Correction of Microplate Data from High-Throughput Screening

      • Yuhong Wang, Ruili Huang

      Pages 123-134

   4. CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprints

      • Alexander Sedykh

      Pages 135-141

   5. Accounting Artifacts in High-Throughput Toxicity Assays

      • Jui-Hua Hsieh

      Pages 143-152

   6. Accessing the High-Throughput Screening Data Landscape

      • Daniel P. Russo, Hao Zhu

      Pages 153-159

This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assays, and computational techniques to analyze HTS data are all examined. Written for the highly successful Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, High Throughput Screening Assays in Toxicology serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailing animal models.

• Chemical toxicity testing
• HTS assays
• Computational techniques
• In vitro and In vivo
• Toxicology assessment tools
• HTS data analysis techniques

• Department of Chemistry Rutgers Center for Computational and Integrative Biology, Rutgers University, Camden, USA

  Hao Zhu

• National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, USA

  Menghang Xia

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