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Methods in Molecular Biology

The Tumor Microenvironment

Methods and Protocols

Editors: Ursini-Siegel, Josie, Beauchemin, Nicole (Eds.)

  • Includes cutting-edge methods and protocols for tumor microenvironment research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-3801-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-3799-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology.  Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.

Table of contents (23 chapters)

  • Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible

    Mori, Hidetoshi (et al.)

    Pages 1-12

  • Laser Capture Microdissection as a Tool to Study Tumor Stroma

    Bertos, Nicholas R. (et al.)

    Pages 13-25

  • Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging

    Willetts, Lian (et al.)

    Pages 27-37

  • Studies on the Tumor Vasculature and Coagulant Microenvironment

    D’Asti, Esterina (et al.)

    Pages 39-58

  • A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment

    Jiménez-Torres, José A. (et al.)

    Pages 59-69

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-3801-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-3799-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Tumor Microenvironment
Book Subtitle
Methods and Protocols
Editors
  • Josie Ursini-Siegel
  • Nicole Beauchemin
Series Title
Methods in Molecular Biology
Series Volume
1458
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3801-8
DOI
10.1007/978-1-4939-3801-8
Hardcover ISBN
978-1-4939-3799-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 356
Number of Illustrations and Tables
19 b/w illustrations, 19 illustrations in colour
Topics